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Literature summary extracted from
- Purification and characterization of 1,2-alpha-mannosidase of Aspergillus oryzae (1982), J. Biochem., 91, 1971-1979.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | L-cysteine | slight stimulation at 1 or 10 mM | Aspergillus oryzae |  |
| 3.2.1.24 | NaN3 | 70% stimulation at 10 mM | Aspergillus oryzae | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Ag+ | 52% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Ba2+ | 10 mM; 78% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Cd2+ | 10 mM, activation at 1 mM; 54% inhibition at 10 mM, slight stimulation at 1 mM | Aspergillus oryzae | |
| 3.2.1.24 | Co2+ | 51% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Cu2+ | 10 mM; 41% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | D-mannono-1,4-lactone | 36% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | D-mannono-gamma-lactone | - |
Aspergillus oryzae | |
| 3.2.1.24 | EDTA | slight inhibition at 1 mM | Aspergillus oryzae | |
| 3.2.1.24 | Fe2+ | 58% inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Fe3+ | 43% inhibition at 1 mM | Aspergillus oryzae | |
| 3.2.1.24 | Hg2+ | complete inhibition at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | iodoacetic acid | - |
Aspergillus oryzae | |
| 3.2.1.24 | methyl-alpha-mannoside | not | Aspergillus oryzae | |
| 3.2.1.24 | additional information | no inhibition by L-cysteine | Aspergillus oryzae | |
| 3.2.1.24 | Na+ | 1 mM, weak, not at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Zn2+ | 55% inhibition at 10 mM | Aspergillus oryzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 0.57 | - |
2-O-alpha-D-mannopyranosyl-D-mannopyranose | - |
Aspergillus oryzae | |
| 3.2.1.24 | 0.57 | - |
2-O-alpha-D-Mannopyranosyl-D-mannose | 37°C, pH 5.3 | Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Cd2+ | activation, 1 mM, inhibits at 10 mM | Aspergillus oryzae | |
| 3.2.1.24 | Mg2+ | not | Aspergillus oryzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 49000 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | Aspergillus oryzae | - |
2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | Aspergillus oryzae | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | Aspergillus oryzae | contains only alpha-1,2-linked mannose residues, highly specific on alpha-1,2-linked mannooligosaccharides | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | mannan + H2O | Aspergillus oryzae | from yeast | alpha-D-mannose + ? | only mannose librated | ? | |
| 3.2.1.24 | ribonuclease B + H2O | Aspergillus oryzae | from bovine pancreas | oligosaccharide + alpha-D-mannopyranose + ? | single oligosaccharide component | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | - |
Aspergillus oryzae |
| 3.2.1.24 | further purification of TAKA-diastase, to homogeneity | Aspergillus oryzae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | commercial preparation | TAKA diastase, crude enzyme preparation | Aspergillus oryzae | - |
| 3.2.1.24 | commercial preparation | Taka-diastase, enzyme-product from Sankyo Co. | Aspergillus oryzae | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.24 | 4°C, pH 4.5-6, at least 6 months | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | - |
Aspergillus oryzae | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | - |
Aspergillus oryzae | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannose + H2O | - |
Aspergillus oryzae | D-mannose | - |
? | |
| 3.2.1.24 | baker's yeast mannan + H2O | releases 9.2% of total mannose | Aspergillus oryzae | ? + D-mannose | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | from ovalbumin | Aspergillus oryzae | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | contains only alpha-1,2-linked mannose residues, highly specific on alpha-1,2-linked mannooligosaccharides | Aspergillus oryzae | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | highly active on alpha(1-2) linked mannooligosaccharides | Aspergillus oryzae | (Man)6(GlcNAc)2 + D-mannose | - |
? | |
| 3.2.1.24 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | i.e. GPIV | Aspergillus oryzae | (Man)5(GlcNAc)2-Asn + D-mannose | - |
? | |
| 3.2.1.24 | mannan + H2O | from yeast | Aspergillus oryzae | alpha-D-mannose + ? | only mannose librated | ? | |
| 3.2.1.24 | methyl-2,4,6-tri-O-methyl-D-mannopyranoside + H2O | - |
Aspergillus oryzae | 2,4,6-tri-O-methyl-D-mannopyranose + methanol | - |
? | |
| 3.2.1.24 | additional information | no substrate: 3-O-alpha- and 6-O-alpha-D-mannobioses | Aspergillus oryzae | ? | - |
? | |
| 3.2.1.24 | ribonuclease B + H2O | from bovine pancreas | Aspergillus oryzae | oligosaccharide + alpha-D-mannopyranose + ? | single oligosaccharide component | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 37 | - |
assay at | Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 50 | - |
10% loss of activity after 1 h | Aspergillus oryzae |
| 3.2.1.24 | 50 | - |
1 h, in 0.1 M acetate buffer, pH 5, 10% loss of activity | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.9 | 5.3 | - |
Aspergillus oryzae |
| 3.2.1.24 | 4.9 | 5.3 | 2-O-alpha-D-mannopyranosyl-D-mannose as substrate | Aspergillus oryzae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4 | 5.8 | about half-maximal activity at pH 4 and 5.8, 0.1 M acetate buffer | Aspergillus oryzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.5 | 6 | stable for 6 months at 4°C | Aspergillus oryzae |
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