Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakamoto, N.
    GMP synthetase (Escherichia coli) (1978), Methods Enzymol., 51, 213-218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.2 6-Amino-9-D-psicofuranosylpurine i.e. psicofuranine, irreversible inhibition, dependent on presence of XMP and diphosphate Escherichia coli
6.3.5.2 6-diazo-5-oxo-L-norleucine
-
Escherichia coli
6.3.5.2 guanidine hydrochloride
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.029
-
XMP
-
Escherichia coli
6.3.5.2 0.53
-
ATP
-
Escherichia coli
6.3.5.2 1
-
NH4+ Gln Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 63000
-
2 * 63000, SDS-PAGE, sedimentation equilibrium measurement in 6.0 M guanidine hydrochloride Escherichia coli
6.3.5.2 126000
-
sedimentation equilibrium measurement Escherichia coli
6.3.5.2 128000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Escherichia coli
-
-
-
6.3.5.2 Escherichia coli B96
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.2 9.09
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + Gln identical Km value for NH4+ and Gln Escherichia coli AMP + diphosphate + GMP + Glu
-
?
6.3.5.2 ATP + XMP + Gln identical Km value for NH4+ and Gln Escherichia coli B96 AMP + diphosphate + GMP + Glu
-
?
6.3.5.2 ATP + XMP + NH4+ identical Km value for NH4+ and Gln Escherichia coli AMP + diphosphate + GMP
-
?
6.3.5.2 ATP + XMP + NH4+ identical Km value for NH4+ and Gln Escherichia coli B96 AMP + diphosphate + GMP
-
?

Subunits

EC Number Subunits Comment Organism
6.3.5.2 dimer 2 * 63000, SDS-PAGE, sedimentation equilibrium measurement in 6.0 M guanidine hydrochloride Escherichia coli