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Literature summary extracted from

  • Shigematsu, Y.; Tsukahara, K.; Tanaka, T.; Takeuchi, M.; Ichishima, E.
    Molecular and enzymic properties of 1,2-alpha-D-mannosidase from Aspergillus saitoi (1986), Curr. Microbiol., 13, 43-46.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 1-cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide inhibitory at 25 mM, pseudo-first-order kinetic Aspergillus phoenicis
3.2.1.24 Co2+ slight inhibition at 10 mM Aspergillus phoenicis
3.2.1.24 Cu2+ slight inhibition at 10 mM Aspergillus phoenicis
3.2.1.24 EDTA slight inhibition at 1 mM Aspergillus phoenicis
3.2.1.24 Fe2+ slight inhibition at 10 mM Aspergillus phoenicis
3.2.1.24 iodoacetic acid slight inhibition at 1 mM Aspergillus phoenicis
3.2.1.24 methyl-alpha-D-mannopyranoside 20% inhibition at 10 mM Aspergillus phoenicis
3.2.1.24 Mn2+
-
 Aspergillus phoenicis
3.2.1.113 1-Cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide/glycine methylester
-
 Aspergillus phoenicis
3.2.1.113 Ag+ not Aspergillus phoenicis
3.2.1.113 Co2+
-
 Aspergillus phoenicis
3.2.1.113 Cu2+
-
 Aspergillus phoenicis
3.2.1.113 EDTA weak Aspergillus phoenicis
3.2.1.113 Fe2+
-
 Aspergillus phoenicis
3.2.1.113 iodoacetic acid monoiodoacetic acid, 1 mM, weak Aspergillus phoenicis
3.2.1.113 mannosamine not Aspergillus phoenicis
3.2.1.113 methyl-alpha-mannoside
-
 Aspergillus phoenicis
3.2.1.113 Mn2+
-
 Aspergillus phoenicis
3.2.1.113 additional information no inhibition by 4-nitrophenyl-alpha-mannoside, mannitol, L-mannono-1,4-lactone, PMSF Aspergillus phoenicis
3.2.1.113 PCMB not Aspergillus phoenicis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.67
-
 mannobiose
-
 Aspergillus phoenicis
3.2.1.113 0.67
-
 mannobiose pH 5.0 Aspergillus phoenicis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.113 additional information
-
 amino acid composition Aspergillus phoenicis
3.2.1.113 64000
-
 x * 64000, SDS-PAGE Aspergillus phoenicis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus phoenicis
-
 2 D-mannopyranose
-
 ?
3.2.1.24 TAKA-amylase A + H2O Aspergillus phoenicis from Aspergillus oryzae, hydrolysis of a single Man-1,2-linkage alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Aspergillus phoenicis
-
-
-
3.2.1.113 Aspergillus phoenicis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.113 glycoprotein 19.4% neutral and 3.9% amino sugars Aspergillus phoenicis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
 Aspergillus phoenicis
-
3.2.1.113 culture filtrate
-
 Aspergillus phoenicis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
-
 Aspergillus phoenicis 2 D-mannopyranose
-
 ?
3.2.1.24 mannobiose + H2O
-
 Aspergillus phoenicis ?
-
 ?
3.2.1.24 TAKA-amylase A + H2O from Aspergillus oryzae, hydrolysis of a single Man-1,2-linkage Aspergillus phoenicis alpha-D-mannopyranose + ?
-
 ?
3.2.1.113 baker's yeast mannan + H2O
-
 Aspergillus phoenicis ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O as in Taka-amylase A Aspergillus phoenicis (Man)5(GlcNAc)2 + D-mannose
-
 ?
3.2.1.113 mannobiose + H2O
-
 Aspergillus phoenicis 2 D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 ? x * 64000, SDS-PAGE Aspergillus phoenicis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 30
-
 assay at Aspergillus phoenicis