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Literature summary extracted from
- Alpha-mannosidase from Rhodococcus erythropolis (1988), Methods Enzymol., 160, 620-626.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.24 | increasing thermostability in the presence of 0.1-1 mM CaCl2 | Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Ag+ | moderate inhibition at 1 mM | Rhodococcus erythropolis |  |
| 3.2.1.24 | Cu2+ | strong inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | EDTA | strong inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Fe2+ | nearly complete inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Hg2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Mn2+ | slight inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Ni2+ | moderate inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | p-chloromercuribenzenesulfonic acid | complete inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Pb2+ | moderate inhibition at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Zn2+ | moderate inhibition at 1 mM | Rhodococcus erythropolis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | additional information | - |
Mannan | value in mg/ml | Rhodococcus erythropolis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.24 | extracellular | - |
Rhodococcus erythropolis | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Ca2+ | moderate activation at 1 mM | Rhodococcus erythropolis | |
| 3.2.1.24 | Mg2+ | moderate activation at 1 mM | Rhodococcus erythropolis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 30000 | 32000 | gel filtration, sedimentation equilibrium centrifugation | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | mannan + H2O | Rhodococcus erythropolis | yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues | alpha-D-mannose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Rhodococcus erythropolis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | glycoprotein | 15% carbohydrate content, mannose, glucose, galactose major components | Rhodococcus erythropolis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps, isoelectric focusing | Rhodococcus erythropolis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | cell culture | - |
Rhodococcus erythropolis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 13890 | - |
- |
Rhodococcus erythropolis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.24 | -20°C, 0.2 mg protein/ml, 1 year | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | mannan + H2O | yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues | Rhodococcus erythropolis | alpha-D-mannose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | monomer | 1 * 30000-32000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | mannanase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 45 | - |
- |
Rhodococcus erythropolis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 60 | - |
fully inactivated in 5 min | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 5.8 | - |
in acetate buffer | Rhodococcus erythropolis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.3 | 9 | - |
Rhodococcus erythropolis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.8 | 8 | stable for 5 h at 20°C | Rhodococcus erythropolis |
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Release 2023.1 (January 2023)
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