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Literature summary extracted from

  • Bonay, P.; Hughes, R.C.
    Purification and characterization of a novel broad-specificity (alpha1-2, alpha1-3 and alpha1-6) mannosidase from rat liver (1991), Eur. J. Biochem., 197, 229-238.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.24 stable in the presence of Co2+ at 37°C Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Cu2+ strong inhibition at 1 mM Rattus sp.
3.2.1.24 EDTA strong inhibition at 1 mM Rattus sp.
3.2.1.24 Fe2+ slight inhibition at 1 mM Rattus sp.
3.2.1.24 iodoacetamide slight inhibition at 1 mM Rattus sp.
3.2.1.24 p-chloromercuribenzenesulfonic acid strong inhibition at 1 mM Rattus sp.
3.2.1.24 swainsonine 50% inhibition in the range of 0.3-0.6 mM Rattus sp.
3.2.1.24 Zn2+ strong inhibition at 1 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.065
-
 Man5GlcNAc
-
 Rattus sp.
3.2.1.24 0.11
-
 Man9GlcNAc
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 membrane from microsomal membranes Rattus sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ 20% activation at 1 mM Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 110000
-
 2 * 110000, reducing and non-reducing SDS-PAGE Rattus sp.
3.2.1.24 220000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O Rattus sp.
-
 6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc end products ?
3.2.1.24 Man6GlcNAc + H2O Rattus sp. ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 16.27
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
-
 Rattus sp. 6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc end products ?
3.2.1.24 Man6GlcNAc + H2O ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues Rattus sp. alpha-D-mannopyranose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 dimer 2 * 110000, reducing and non-reducing SDS-PAGE Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6.1 6.5
-
 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.24 0.3 0.6 swainsonine
-
 Rattus sp.