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Literature summary extracted from

  • Vazquez-Reyna, A.B.; Balcazar-Orozco, R.; Flores-Carreon, A.
    Biosynthesis of glycoproteins in Candida albicans: Biochemical characterization of a soluble alpha-mannosidase (1993), FEMS Microbiol. Lett., 106, 321-326.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 1-deoxymannojirimicin 67% inhibition at 1 mM when 4-methylumbelliferyl-alpha-D-mannopyranoside is used, 15% inhibition when p-nitrophenyl-alpha-D-mannopyranoside is used as substrates Candida albicans
3.2.1.24 Ca2+ 53% inhibition at 0.1 mM Candida albicans
3.2.1.24 Cu2+ complete inhibition at 0.1 mM Candida albicans
3.2.1.24 EDTA 37% inhibition at 5 mM Candida albicans
3.2.1.24 EGTA 32% inhibition at 5 mM Candida albicans
3.2.1.24 Mn2+ 58% inhibition at 0.1 mM Candida albicans
3.2.1.24 SDS 85% inhibition at 0.05%, w/v Candida albicans
3.2.1.24 swainsonine 83% inhibition at 0.3 mM if 4-methylumbelliferyl-alpha-D-mannopyranoside is used, hydrolysis of p-nitrophenyl-alpha-D-mannopyranoside only slightly diminished at 0.3 mM Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.1
-
 4-methylumbelliferyl-alpha-D-mannopyranoside
-
 Candida albicans
3.2.1.24 3.3
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 cytosol
-
 Candida albicans 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 417000
-
 gel filtration Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 partial Candida albicans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
 Candida albicans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 0.11
-
 with p-nitrophenyl-alpha-D-mannopyranoside as substrate Candida albicans
3.2.1.24 2200
-
 with 4-methylumbelliferyl-alpha-D-mannopyranoside as substrate Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans p-nitrophenol + alpha-D-mannopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 42
-
-
 Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 37
-
 10% loss of activity after 2.5 h Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6
-
-
 Candida albicans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 5.5
-
 activity lowered by 22% Candida albicans
3.2.1.24 6.8
-
 activity lowered by 27% Candida albicans