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Literature summary extracted from

  • Spector, T.
    GMP synthetase from Ehrlich ascites cells (1978), Methods Enzymol., 51, 219-224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.2 DTT stimulates Mus musculus

General Stability

EC Number General Stability Organism
6.3.5.2 thawing and refreezing does not diminish activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.2 1,N6-Ethenoadenosine 5'-triphosphate
-
 Mus musculus
6.3.5.2 2-mercaptoethanol
-
 Mus musculus
6.3.5.2 6-thioXMP
-
 Mus musculus
6.3.5.2 adenine 9-beta-D-arabinofuranoside 5'-triphosphate
-
 Mus musculus
6.3.5.2 CTP
-
 Mus musculus
6.3.5.2 diphosphate
-
 Mus musculus
6.3.5.2 p-chloromercuribenzoate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.0036
-
 XMP
-
 Mus musculus
6.3.5.2 0.28
-
 ATP
-
 Mus musculus
6.3.5.2 0.68
-
 Gln
-
 Mus musculus
6.3.5.2 36
-
 NH4+
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.2 Mg2+ required Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 85000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.2 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.2 0.0428
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
6.3.5.2 -70°C, stable for at least 1 year Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + 1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine 5-phosphate
-
 Mus musculus AMP + phosphate + 1-ribosyl-4-hydroxy-6-aminopyrazolo[3,4-d]pyrimidine 5'-phosphate
-
 ?
6.3.5.2 ATP + 6-thioXMP + NH4+
-
 Mus musculus AMP + phosphate + 6-thioGMP
-
 ?
6.3.5.2 ATP + 8-azaXMP + NH4+
-
 Mus musculus AMP + phosphate + 8-azaGMP
-
 ?
6.3.5.2 ATP + beta-arabinofuranosyl-XMP
-
 Mus musculus AMP + phosphate + beta-arabinofuranosylGMP
-
 ?
6.3.5.2 ATP + dXMP + NH4+ at 4.3% of the activity relative to XMP Mus musculus AMP + phosphate + dGMP
-
 ?
6.3.5.2 ATP + XMP + Gln higher Km value for NH4+ compared with Gln Mus musculus AMP + diphosphate + GMP + Glu
-
 ?
6.3.5.2 ATP + XMP + NH4+ higher Km value for NH4+ compared with Gln Mus musculus AMP + diphosphate + GMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.2 7.6
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.2 7.4 8.6 more than 80% of maximal activity at pH 7.4 and 8.6 Mus musculus