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Literature summary extracted from

  • Hemscheid, T.; Zenk, M.H.
    Glucosidases involved in indole alkaloid biosynthesis of Catharanthus cell culture (1980), FEBS Lett., 110, 187-191.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 D(+)-gluconic acid delta-lactone enzyme I is much less sensitive than enzyme II Catharanthus roseus
3.2.1.105 strictosidine enzyme I shows substrate inhibition at 1 mM, enzyme II not Catharanthus roseus
3.2.1.105 tryptamine 2.5 mM, 15% inhibition of enzyme I and II Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.1
-
 strictosidine enzyme II Catharanthus roseus
3.2.1.105 0.2
-
 strictosidine enzyme I Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.105 230000
-
 enzyme I, gel filtration Catharanthus roseus
3.2.1.105 450000
-
 above, enzyme II, gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.105 strictosidine Catharanthus roseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Amsonia orientalis
-
 weak activity
-
3.2.1.105 Amsonia salicifolia
-
-
-
3.2.1.105 Catharanthus ovalis
-
-
-
3.2.1.105 Catharanthus pusillus
-
-
-
3.2.1.105 Catharanthus roseus
-
-
-
3.2.1.105 Catharanthus trichophyllus
-
-
-
3.2.1.105 Rauvolfia vomitoria
-
 weak activity
-
3.2.1.105 Vinca major
-
 weak activity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 strictosidine
-
 Catharanthus roseus ?
-
 ?
3.2.1.105 vincoside + H2O hydrolyzed at less than 10% of strictosidine Catharanthus roseus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.105 30
-
-
 Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.105 6 6.4 enzyme I and II Catharanthus roseus