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Literature summary extracted from

  • Luijendijk, T.J.C.; Nowak, A.; Verpoorte, R.
    Strictosidine glucosidase from suspension cultured cells of Tabernaemontana divaricata (1996), Phytochemistry, 41, 1451-1456.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.105 highly increased sensitivity for freezing and thawing after separation over Superose 6 Tabernaemontana divaricata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 D(+)-gluconic acid delta-lactone some inhibition at a concentration of 10 mM and higher Tabernaemontana divaricata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.25 0.33 strictosidine enzyme form SGIII Tabernaemontana divaricata
3.2.1.105 1
-
 strictosidine above, enzyme form SGII Tabernaemontana divaricata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.105 22000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
3.2.1.105 31000
-
 enzyme form SGIII, non-denaturing PAGE Tabernaemontana divaricata
3.2.1.105 187000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
3.2.1.105 190000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
3.2.1.105 670000
-
 above, enzyme form SGI, gel filtration Tabernaemontana divaricata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Tabernaemontana divaricata
-
 cell line 59 and cell line 6B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.105 partial, three enzyme forms: SGI, SGII and SGIII Tabernaemontana divaricata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.105 cell suspension culture
-
 Tabernaemontana divaricata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.105 additional information
-
-
 Tabernaemontana divaricata

Storage Stability

EC Number Storage Stability Organism
3.2.1.105 -80°C, stable for 6 months Tabernaemontana divaricata
3.2.1.105 4°C, 24 h, stable Tabernaemontana divaricata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 additional information no activity with secologanin, strictosamide or p-nitrophenyl-beta-D-glucopyranoside Tabernaemontana divaricata ?
-
 ?
3.2.1.105 strictosidine + H2O
-
 Tabernaemontana divaricata D-glucose + strictosidine aglycone the major intermediate after rearrangement of the strictosidine aglycone is thought to be 21-hydroxyajmalicine ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.105 4.5 5 enzyme form SGI, a second pH-optimum at pH 7.0 Tabernaemontana divaricata
3.2.1.105 7
-
 enzyme form SGI, a second pH-optimum at pH 4.5-5.0 Tabernaemontana divaricata