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Literature summary extracted from

  • Brigance, W.T.; Barlowe, C.; Graham, T.R.
    Organization of the yeast golgi complex into at least four functionally distinct compartments (2000), Mol. Biol. Cell, 11, 171-182.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.101 analysis used to define kinetics of mannosylation of pro-alpha-factor, pro-alphaf, to distinguish alpha-1,6-mannosylated and alpha-1,2-mannosylated forms of glycoproteins Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.101 extracellular
-
 Niallia circulans
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.101 Niallia circulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.101 partial Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.101 0.45
-
-
 Niallia circulans

Storage Stability

EC Number Storage Stability Organism
3.2.1.101 4°C, 1 ml aliquots, 0.1 M citrate-phosphate buffer, pH 6.0, or 0.1 M potassium phosphate buffer, pH 6.8, 1 mM CaCl2, 1 year, 80% original activity retained Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.101 alpha-1,6-D-mannan + H2O
-
 Niallia circulans D-mannose + alpha-1,6-D-mannobiose
-
 ?
3.2.1.101 alpha-1,6-D-mannooligosaccharide + H2O unbranched alpha-1,6 outer chain of N-linked oligosaccharides Niallia circulans mannose + alpha-1,6-D-mannobiose
-
 ?
3.2.1.101 alpha-1,6-mannosylated pro-alpha-factor + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.101 additional information no activity with alpha-1,2-mannosylated pro-alpha-factor Niallia circulans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.101 endoM
-
 Niallia circulans