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Literature summary extracted from

  • Masue, M.; Sukegawa, K.; Orii, T.; Hashimoto, T.
    N-Acetylgalactosamine-6-sulfate sulfatase in human placenta: purification and characteristics (1991), J. Biochem., 110, 965-970.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.6.4 freezing and thawing five times do not decrease the enzyme activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.4 KCl
-
 Homo sapiens
3.1.6.4 Na2SO4
-
 Homo sapiens
3.1.6.4 NaCl
-
 Homo sapiens
3.1.6.4 NaH2PO4
-
 Homo sapiens
3.1.6.4 phosphate
-
 Homo sapiens
3.1.6.4 SO42-
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.4 0.008
-
 galactosamine 6-sulfate
-
 Homo sapiens
3.1.6.4 0.013
-
 Galactose 6-sulfate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.4 15000
-
 x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens
3.1.6.4 40000
-
 x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens
3.1.6.4 120000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.6.4 glycoprotein the 15000 Da subunit is a glycoprotein Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.4 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.4 2.13
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.6.4 -20°C, 2 months, 5% loss of activity Homo sapiens
3.1.6.4 4°C, 2 months, 20% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.4 beta-N-acetyl-D-galactosamine-6-sulfate-(1-4)-beta-D-glucuronic acid-(1-3)-N-acetyl-D-galactosaminitol 6-sulfate + H2O
-
 Homo sapiens sulfate + ?
-
 ?
3.1.6.4 galactosamine 6-sulfate + H2O
-
 Homo sapiens galactosamine + sulfate
-
 ?
3.1.6.4 galactose 6-sulfate + H2O
-
 Homo sapiens galactose + sulfate
-
 ?
3.1.6.4 N-acetylgalactosamine 6-sulfate + H2O
-
 Homo sapiens N-acetylgalactosamine + sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.4 ? x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.4 3.8 4 hydrolysis of N-acetylgalactosamine 6-sulfate or galactose 6-sulfate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.4 3.3 4.2 pH 3.0: about 45% of maximal activity, pH 4.2: about 55% of maximal activity, hydrolysis of N-acetylgalactosamine 6-sulfate, 0.02 M acetate buffer Homo sapiens