Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Habuchi, H.; Tsuji, M.; Nakanishi, Y.; Suzuki, S.
    Separation and properties of five glycosaminoglycan sulfatases from rat skin (1979), J. Biol. Chem., 254, 7570-7578.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.12 KCl at 10 mM Rattus norvegicus
3.1.6.12 Mg2+
-
 Rattus norvegicus
3.1.6.12 Mn2+
-
 Rattus norvegicus
3.1.6.12 NaCl at 10 mM Rattus norvegicus
3.1.6.12 SO42-
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.4 0.027
-
 GalNAc-4-SO4-GlcUA-GalNAc-4-SO4
-
 Rattus norvegicus
3.1.6.4 0.03
-
 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)2
-
 Rattus norvegicus
3.1.6.4 0.049
-
 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)3
-
 Rattus norvegicus
3.1.6.4 0.064
-
 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)4
-
 Rattus norvegicus
3.1.6.12 0.027
-
 N-acetylgalactosamine 4-sulfate-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate
-
 Rattus norvegicus
3.1.6.12 0.03
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2
-
 Rattus norvegicus
3.1.6.12 0.049
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3
-
 Rattus norvegicus
3.1.6.12 0.064
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.12 lysosome
-
 Rattus norvegicus 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O Rattus norvegicus acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.4 Rattus norvegicus
-
-
-
3.1.6.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.12
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.4 skin
-
 Rattus norvegicus
-
3.1.6.12 skin
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.4 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)2 + H2O
-
 Rattus norvegicus sulfate + ?
-
 ?
3.1.6.4 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)3 + H2O
-
 Rattus norvegicus sulfate + ?
-
 ?
3.1.6.4 GalNAc-4-SO4-(GlcUA-GalNAc-4-SO4)4 + H2O
-
 Rattus norvegicus sulfate + ?
-
 ?
3.1.6.4 GalNAc-4-SO4-GlcUA-GalNAc-4-SO4 + H2O
-
 Rattus norvegicus sulfate + ?
-
 ?
3.1.6.12 chondroitin 4-sulfate + H2O
-
 Rattus norvegicus chondroitin + sulfate
-
 ?
3.1.6.12 chondroitin 4-sulfate-heptasaccharide + H2O
-
 Rattus norvegicus chondroitin 4-sulfate-heptasaccharide + sulfate
-
 ?
3.1.6.12 dermatan sulfate + H2O
-
 Rattus norvegicus dermatan + sulfate
-
 ?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetyl-D-galactosamine + sulfate
-
 ?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Rattus norvegicus N-acetyl-D-galactosamine units + sulfate
-
 ?
3.1.6.12 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2 + sulfate
-
 ?
3.1.6.12 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine)3 + sulfate
-
 ?
3.1.6.12 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4 + sulfate
-
 ?
3.1.6.12 N-acetylgalactosamine 4-sulfate-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetylgalactosamine-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate + sulfate
-
 ?
3.1.6.12 N-acetylglucosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetylglucosamine + sulfate
-
 ?
3.1.6.12 p-nitrocatechol sulfate + H2O
-
 Rattus norvegicus p-nitrocatechol + sulfate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.12 4 4.8 substrate-dependent Rattus norvegicus