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Literature summary extracted from

  • Iwamori, M.; Moser, H.W.; Kishimoto, Y.
    Solubilization and partial purification of steroid sulfatase from rat liver: characterization of estrone sulfatase (1976), Arch. Biochem. Biophys., 174, 199-208.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.6.2 lyophilization, deactivation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.2 (NH4)2SO4 slight Rattus norvegicus
3.1.6.2 AgNO3 slight Rattus norvegicus
3.1.6.2 CaCl2 slight Rattus norvegicus
3.1.6.2 EDTA 10% inhibition at 1 mM Rattus norvegicus
3.1.6.2 KBr slight Rattus norvegicus
3.1.6.2 KCl slight Rattus norvegicus
3.1.6.2 KCN slight Rattus norvegicus
3.1.6.2 LiCl slight Rattus norvegicus
3.1.6.2 Metabisulfite
-
 Rattus norvegicus
3.1.6.2 MgCl2 slight Rattus norvegicus
3.1.6.2 Na-metaborate
-
 Rattus norvegicus
3.1.6.2 Na-tetraborate
-
 Rattus norvegicus
3.1.6.2 Na2SO3 slight Rattus norvegicus
3.1.6.2 Na2SO4 slight Rattus norvegicus
3.1.6.2 NaCl slight Rattus norvegicus
3.1.6.2 NaF slight Rattus norvegicus
3.1.6.2 NaHSO3 slight Rattus norvegicus
3.1.6.2 SDS
-
 Rattus norvegicus
3.1.6.2 sodium arsenite slight Rattus norvegicus
3.1.6.2 sodium deoxycholate
-
 Rattus norvegicus
3.1.6.2 sodium taurocholate
-
 Rattus norvegicus
3.1.6.2 Steroids overview, e.g. estrone, estradiol, C21 steroids, C19 steroids Rattus norvegicus
3.1.6.2 Sulfate esters overview, e.g. p-nitrophenyl sulfate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.2 microsome membrane-bound Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.2 130000
-
 gel filtration Rattus norvegicus
3.1.6.2 130000
-
 estrone sulfatase is identical to arylsulfatase C but not to sulfatases which hydrolyse dehydroepiabdrosterone, testosterone, pregnenolone, and cholesterol Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.2 steroid sulfates + H2O Rattus norvegicus also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site steroid + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.2 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.2 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.6.2 4oC, pH 7.4, imidazol buffer, several months, small loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.2 cholesterol 3-sulfate + H2O
-
 Rattus norvegicus cholesterol + sulfate
-
 ?
3.1.6.2 dehydroandrosterone 3-sulfate + H2O
-
 Rattus norvegicus dehydroandrosterone + sulfate
-
 ?
3.1.6.2 dehydroepiandrosterone 3-sulfate + H2O
-
 Rattus norvegicus dehydroepiandrosterone + sulfate
-
 ?
3.1.6.2 estrone sulfate + H2O
-
 Rattus norvegicus estrone + sulfate
-
 ?
3.1.6.2 additional information
-
 Rattus norvegicus ?
-
 ?
3.1.6.2 p-nitrophenyl-sulfate + H2O arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein Rattus norvegicus p-nitrophenol + sulfate
-
 ?
3.1.6.2 pregnenolone 3-sulfate + H2O
-
 Rattus norvegicus pregnenolone + sulfate
-
 ?
3.1.6.2 steroid sulfates + H2O also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site Rattus norvegicus steroid + sulfate
-
 ?
3.1.6.2 testosterone 3-sulfate + H2O
-
 Rattus norvegicus testosterone + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.2 More arylsulfatase activity, E.C. 3.1.6.1, and various steroid sulfatase activities, E.C. 3.1.6.1, are functions of the same protein Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.6.2 70
-
 10 min, 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.2 6.6
-
 dehydroepiandrosterone 3-sulfate Rattus norvegicus
3.1.6.2 8
-
 esterone 3-sulfate Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.2 5 8.5 pH 5: about 25% of activity maximum, pH 8.5: about 15% of activity maximum, dehydroepiandrosterone 3-sulfate Rattus norvegicus