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Literature summary extracted from

  • Daniel, W.L.; Ruoff, B.M.; Thompson, D.B.
    Purification and properties of arylsulfatase B from high- and low-activity mouse strains (1985), Biochem. Genet., 23, 771-786.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.12 Ag+ not Mus musculus
3.1.6.12 Ba2+ not; slight Mus musculus
3.1.6.12 Na2HPO4
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.12 1.3
-
p-nitrocatechol sulfate
-
Mus musculus
3.1.6.12 4
-
4-Methylumbelliferyl sulfate pH 5.5 Mus musculus
3.1.6.12 5.6
-
4-Methylumbelliferyl sulfate
-
Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.12 lysosome
-
Mus musculus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.12 50000
-
1 * 50000, SDS-PAGE, gel filtration Mus musculus
3.1.6.12 55000
-
gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O Mus musculus acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.12 Mus musculus
-
3 strains: C57BL/6J, SWR/J and A/J
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.12
-
Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.12 liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.12 30.7
-
strain A/J Mus musculus
3.1.6.12 59.55
-
strain SWR/J Mus musculus
3.1.6.12 67.53
-
strain C57BL/6J Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.12 4-methylumbelliferyl sulfate + H2O
-
Mus musculus 4-methylumbelliferone + sulfate
-
?
3.1.6.12 chondroitin 4-sulfate + H2O
-
Mus musculus chondroitin + sulfate
-
?
3.1.6.12 chondroitin 4-sulfate-heptasaccharide + H2O
-
Mus musculus chondroitin 4-sulfate-heptasaccharide + sulfate
-
?
3.1.6.12 dermatan sulfate + H2O
-
Mus musculus dermatan + sulfate
-
?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate + H2O
-
Mus musculus N-acetyl-D-galactosamine + sulfate
-
?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Mus musculus N-acetyl-D-galactosamine units + sulfate
-
?
3.1.6.12 p-nitrocatechol sulfate + H2O enzymes catalyze in addition to reaction of EC 3.1.6.12 also arylsulfatase reaction , see also EC 3.1.6.1 Mus musculus p-nitrocatechol + sulfate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.6.12 monomer 1 * 50000, SDS-PAGE, gel filtration Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.6.12 60
-
pH 5.9 Mus musculus
3.1.6.12 60
-
20 min Mus musculus
3.1.6.12 65
-
19 min Mus musculus
3.1.6.12 65
-
t1/2 of A/J enzyme: 20 min Mus musculus
3.1.6.12 65
-
t1/2 of SWR/J enzyme: 46 min Mus musculus