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Literature summary extracted from
- Characterization and partial purification of arylsulfatase from the seminal plasma of the sea urchin, Strongylocentrotus intermedius (1980), Arch. Biochem. Biophys., 201, 216-223.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.6.1 | several cycles of freezing and thawing cause a marked reduction of activity | Strongylocentrotus intermedius |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.1 | Cu2+ | - |
Strongylocentrotus intermedius |  |
| 3.1.6.1 | EDTA | - |
Strongylocentrotus intermedius | |
| 3.1.6.1 | Fe3+ | - |
Strongylocentrotus intermedius | |
| 3.1.6.1 | iodoacetate | - |
Strongylocentrotus intermedius | |
| 3.1.6.1 | Zn2+ | - |
Strongylocentrotus intermedius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.1 | 0.5 | - |
Ascorbate 2-sulfate | - |
Strongylocentrotus intermedius | |
| 3.1.6.1 | 0.53 | - |
4-nitrophenyl sulfate | - |
Strongylocentrotus intermedius | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.1 | Ba2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
| 3.1.6.1 | Ca2+ | required | Strongylocentrotus intermedius | |
| 3.1.6.1 | KCl | stimulates | Strongylocentrotus intermedius | |
| 3.1.6.1 | Mg2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
| 3.1.6.1 | NaCl | stimulates | Strongylocentrotus intermedius | |
| 3.1.6.1 | Sr2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.1 | Strongylocentrotus intermedius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.1 | partial | Strongylocentrotus intermedius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.6.1 | seminal plasma | - |
Strongylocentrotus intermedius | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 1.5 | - |
- |
Strongylocentrotus intermedius |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.6.1 | -20°C, stable for more than 6 months | Strongylocentrotus intermedius |
| 3.1.6.1 | 0°C, without freezing, 72 h, inactivation | Strongylocentrotus intermedius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.1 | 4-nitrophenyl sulfate + H2O | - |
Strongylocentrotus intermedius | 4-nitrophenol + sulfate | - |
? | |
| 3.1.6.1 | ascorbic acid 2-sulfate + H2O | - |
Strongylocentrotus intermedius | ascorbic acid + sulfate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 60 | - |
40 min, 70% loss of activity | Strongylocentrotus intermedius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 6.8 | - |
- |
Strongylocentrotus intermedius |
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