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Literature summary extracted from

  • Kean, E.L.; Bighouse, K.J.
    Cytidine 5-monophosphosialic acid hydrolase. Subcellular location and properties (1974), J. Biol. Chem., 249, 7813-7823.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.40 2-mercaptoethanol 46% inhibition at 25 mM Rattus norvegicus
3.1.4.40 5,5'-dithiobis(2-nitrobenzoic acid) 16.3% inhibition at 25 mM Rattus norvegicus
3.1.4.40 cAMP 19% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 CDP 64% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 CMP 37% inhibition at 0.86 mM, 96% inhibition at 3.8 mM Rattus norvegicus
3.1.4.40 CTP 84% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 Cu2+ 38% reduction of enzyme activity at 10 mM Rattus norvegicus
3.1.4.40 cysteine 80% inhibition at 25 mM Rattus norvegicus
3.1.4.40 cytidine 6.9% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 diphosphate 41% inhibition at 0.86 mM, 82% inhibition at 6.5 Rattus norvegicus
3.1.4.40 dithiothreitol 84% inhibition by 25 mM reduced dithiothreitol, 0% inhibition by 25 mM oxidized dithiotreitol Rattus norvegicus
3.1.4.40 EDTA 50% inhibition at 0.2 mM Rattus norvegicus
3.1.4.40 GDPglucose competitive, 83% inhibition Rattus norvegicus
3.1.4.40 GDPmannose competitive, 85% inhibition Rattus norvegicus
3.1.4.40 glutathione 62% inhibition by 25 mM reduced glutathione, 15% inhibition by 25 mM oxidized glutathione Rattus norvegicus
3.1.4.40 N-ethylmaleimide 5.8% inhibition at 25 mM Rattus norvegicus
3.1.4.40 p-chloromercuribenzoate 7.2% inhibition at 25 mM Rattus norvegicus
3.1.4.40 TDPglucose competitive, 70% inhibition Rattus norvegicus
3.1.4.40 UDP 76% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 UDP-N-acetylglucosamine competitive, 58% inhibition Rattus norvegicus
3.1.4.40 UDPgalactose 31% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 UDPgalacturonic acid competitive, 79% inhibition Rattus norvegicus
3.1.4.40 UDPglucose competitive, 70% inhibition Rattus norvegicus
3.1.4.40 UDPglucuronic acid competitive, 76% inhibition Rattus norvegicus
3.1.4.40 UDPmannose competitive, 61% inhibition Rattus norvegicus
3.1.4.40 UMP 26% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 uridine 7.5% inhibition at 0.86 mM Rattus norvegicus
3.1.4.40 UTP 83% inhibition at 0.86 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.40 0.4
-
 CMP-N-acylneuraminate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.40 microsome
-
 Rattus norvegicus
-
-
3.1.4.40 nucleus
-
 Rattus norvegicus 5634
-
3.1.4.40 plasma membrane activity in subfractions Rattus norvegicus 5886
-
3.1.4.40 plasma membrane of liver cells Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.40 Ca2+ restores 20% of initial enzymatic activity when inhibited by EDTA Rattus norvegicus
3.1.4.40 Mg2+ restores 8% of initial enzymatic activity when inhibited by EDTA Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.40 CMP-N-acylneuraminate + H2O Rattus norvegicus
-
 ?
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.40 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.40
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.40 blood
-
 Rattus norvegicus
-
3.1.4.40 bone
-
 Rattus norvegicus
-
3.1.4.40 brain
-
 Rattus norvegicus
-
3.1.4.40 heart
-
 Rattus norvegicus
-
3.1.4.40 intestinal mucosa
-
 Rattus norvegicus
-
3.1.4.40 kidney
-
 Rattus norvegicus
-
3.1.4.40 liver
-
 Rattus norvegicus
-
3.1.4.40 muscle skeletal Rattus norvegicus
-
3.1.4.40 pancreas
-
 Rattus norvegicus
-
3.1.4.40 retina
-
 Rattus norvegicus
-
3.1.4.40 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.40 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.4.40 -20°C, plasma membranes, 1 year, microsomes, 3 months, no loss of activity Rattus norvegicus
3.1.4.40 0°C, for several days no loss of activity, by the 25th day 42% of initial activity remains Rattus norvegicus
3.1.4.40 repeated freezing and thawing, no influence of enzyme activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.40 CMP-N-acylneuraminate + H2O
-
 Rattus norvegicus CMP + N-acylneuraminate
-
 ir
3.1.4.40 CMP-N-acylneuraminate + H2O
-
 Rattus norvegicus ?
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.40 9
-
 in Tris-HCl buffer Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.40 0.2
-
 EDTA
-
 Rattus norvegicus