BRENDA - Enzyme Database

Purification and characterisation of rape seed phospholipase D

Novotna, Z.; Kas, J.; Daussant, J.; Sajdok, J.; Valentova, O.; Plant Physiol. Biochem. 37, 531-537 (1999)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
SDS
-
Brassica napus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1
4
phosphatidylcholine
depends on SDS
Brassica napus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Brassica napus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
90000
105000
gel filtration, SDS-PAGE
Brassica napus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Brassica napus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Brassica napus
-
Winter rape
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Brassica napus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
seed
-
Brassica napus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
135458
Brassica napus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135458
Brassica napus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135458
Brassica napus
phosphatidic acid + alcohol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
6
-
Brassica napus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
4.5
7
-
Brassica napus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
SDS
-
Brassica napus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1
4
phosphatidylcholine
depends on SDS
Brassica napus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Brassica napus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
90000
105000
gel filtration, SDS-PAGE
Brassica napus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Brassica napus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Brassica napus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
seed
-
Brassica napus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
135458
Brassica napus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135458
Brassica napus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135458
Brassica napus
phosphatidic acid + alcohol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
6
-
Brassica napus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
4.5
7
-
Brassica napus