BRENDA - Enzyme Database

Purification and properties of phospholipase D from Streptomyces antibioticus

Shimbo, K.; Iwasaki,Y.; Yamane, T.; Ina,K.; Biosci. Biotechnol. Biochem. 57, 1946-1948 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
2-mercaptoethanol
-
Streptomyces antibioticus
3.1.4.4
dithiothreitol
-
Streptomyces antibioticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
64000
-
chromatofocusing, SDS-PAGE
Streptomyces antibioticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Streptomyces antibioticus
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Streptomyces antibioticus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Streptomyces antibioticus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Streptomyces antibioticus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces antibioticus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
1437
-
-
Streptomyces antibioticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + H2O
-
135456
Streptomyces antibioticus
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-arabinose
-
135456
Streptomyces antibioticus
phosphatidylarabinose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-fructose
-
135456
Streptomyces antibioticus
phosphatidylfructose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-galactose
-
135456
Streptomyces antibioticus
phosphatidylgalactose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-glucose
-
135456
Streptomyces antibioticus
phosphatidylglucose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-mannose
-
135456
Streptomyces antibioticus
phosphatidylmannose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-xylose
-
135456
Streptomyces antibioticus
phosphatidylxylose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + diethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidyldiethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + diethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
diethyleneglycol dimethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidylethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
ethyleneglycol monomethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135456
Streptomyces antibioticus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-sorbose
-
135456
Streptomyces antibioticus
phosphatidylsorbose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + triethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidyltriethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + triethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
triethyleneglycol trimethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135456
Streptomyces antibioticus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
r
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135456
Streptomyces antibioticus
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135456
Streptomyces antibioticus
phosphatidic acid + alcohol
-
-
-
?
3.1.4.4
sphingomyelin + H2O
-
135456
Streptomyces antibioticus
N-acylsphingosylphosphate + choline
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
60
70
-
Streptomyces antibioticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
-
Streptomyces antibioticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
4
8
stable after 2 h at 37°C
Streptomyces antibioticus
3.1.4.4
5.5
-
50% loss of activity after 30 min at 70°C
Streptomyces antibioticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
2-mercaptoethanol
-
Streptomyces antibioticus
3.1.4.4
dithiothreitol
-
Streptomyces antibioticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
64000
-
chromatofocusing, SDS-PAGE
Streptomyces antibioticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Streptomyces antibioticus
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Streptomyces antibioticus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Streptomyces antibioticus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces antibioticus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
1437
-
-
Streptomyces antibioticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + H2O
-
135456
Streptomyces antibioticus
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-arabinose
-
135456
Streptomyces antibioticus
phosphatidylarabinose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-fructose
-
135456
Streptomyces antibioticus
phosphatidylfructose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-galactose
-
135456
Streptomyces antibioticus
phosphatidylgalactose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-glucose
-
135456
Streptomyces antibioticus
phosphatidylglucose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-mannose
-
135456
Streptomyces antibioticus
phosphatidylmannose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-xylose
-
135456
Streptomyces antibioticus
phosphatidylxylose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + diethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidyldiethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + diethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
diethyleneglycol dimethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidylethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
ethyleneglycol monomethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135456
Streptomyces antibioticus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-sorbose
-
135456
Streptomyces antibioticus
phosphatidylsorbose + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + triethyleneglycol
-
135456
Streptomyces antibioticus
phosphatidyltriethyleneglycol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + triethyleneglycol monomethyl ester
-
135456
Streptomyces antibioticus
triethyleneglycol trimethyl phosphatidic acid + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135456
Streptomyces antibioticus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
r
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135456
Streptomyces antibioticus
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135456
Streptomyces antibioticus
phosphatidic acid + alcohol
-
-
-
?
3.1.4.4
sphingomyelin + H2O
-
135456
Streptomyces antibioticus
N-acylsphingosylphosphate + choline
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
60
70
-
Streptomyces antibioticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
-
Streptomyces antibioticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
4
8
stable after 2 h at 37°C
Streptomyces antibioticus
3.1.4.4
5.5
-
50% loss of activity after 30 min at 70°C
Streptomyces antibioticus