BRENDA - Enzyme Database

Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum

Ogino, C.; Negi, Y.; Matsumiya, T.; Nakaoka, K.; Kondo, A.; Kuroda, S.; Tokuyama, S.; Kikkawa, U.; Yamane, T.; Fukuda, H.; J. Biochem. 125, 263-269 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces cinnamoneus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Al3+
activation of transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Mn2+
substrate specific
Streptomyces cinnamoneus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Al3+
activation of transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Ca2+
stimulates only transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Mn2+
substrate specific
Streptomyces cinnamoneus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
54000
-
gel filtration, SDS-PAGE
Streptomyces cinnamoneus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Streptomyces cinnamoneus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Streptomyces cinnamoneus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Streptomyces cinnamoneus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces cinnamoneus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.4
468
-
-
Streptomyces cinnamoneus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.4
55C, pH 7.4, 24 h, 100% loss of activity
Streptomyces cinnamoneus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135455
Streptomyces cinnamoneus
phosphatidic acid + alcohol
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.4
50
-
-
Streptomyces cinnamoneus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Streptomyces cinnamoneus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces cinnamoneus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Al3+
activation of transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Mn2+
substrate specific
Streptomyces cinnamoneus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Al3+
activation of transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Ca2+
stimulates only transphosphatidylation
Streptomyces cinnamoneus
3.1.4.4
Mn2+
substrate specific
Streptomyces cinnamoneus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
54000
-
gel filtration, SDS-PAGE
Streptomyces cinnamoneus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Streptomyces cinnamoneus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Streptomyces cinnamoneus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces cinnamoneus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.4
468
-
-
Streptomyces cinnamoneus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.4
55C, pH 7.4, 24 h, 100% loss of activity
Streptomyces cinnamoneus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135455
Streptomyces cinnamoneus
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135455
Streptomyces cinnamoneus
phosphatidic acid + alcohol
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.4
50
-
-
Streptomyces cinnamoneus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Streptomyces cinnamoneus