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Literature summary extracted from

  • Okamura, S.; Yamashita, S.
    Purification and characterization of phosphatidylcholine phospholipase D from pig lung (1994), J. Biol. Chem., 269, 31207-31213.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 arachidonic acid only in presence of Mg2+ Sus scrofa
3.1.4.4 linoleic acid only in presence of Mg2+ Sus scrofa
3.1.4.4 lysophosphatidic acid
-
 Sus scrofa
3.1.4.4 oleic acid only in presence of Mg2+ Sus scrofa
3.1.4.4 phosphatidylinositol 4,5-bisphosphate
-
 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 lysophosphatidylcholine
-
 Sus scrofa
3.1.4.4 N-ethylmaleimide
-
 Sus scrofa
3.1.4.4 palmitic acid
-
 Sus scrofa
3.1.4.4 phosphatidic acid
-
 Sus scrofa
3.1.4.4 phosphatidylethanolamine
-
 Sus scrofa
3.1.4.4 phosphatidylinositol
-
 Sus scrofa
3.1.4.4 stearic acid
-
 Sus scrofa
3.1.4.4 sucrose monolaurate
-
 Sus scrofa
3.1.4.4 Triton X-100
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.8
-
 phosphatidylcholine
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ micromolar levels for maximal activity Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 190000
-
 gel filtration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + alcohol Sus scrofa transphosphaditylation phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O Sus scrofa phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 complete to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 lung
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 3.67
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + ethanol
-
 Sus scrofa phosphatidylethanol + choline
-
 ?
3.1.4.4 phosphatidylcholine + ethanolamine
-
 Sus scrofa phosphatidylethanolamine + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Sus scrofa 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylinositol + H2O
-
 Sus scrofa 1,2-diacylglycerophosphate + inositol
-
 ?
3.1.4.4 phospholipid + alcohol transphosphaditylation Sus scrofa phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Sus scrofa phosphatidic acid + alcohol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 6.6
-
-
 Sus scrofa