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Literature summary extracted from
- Improved purification and biochemical characterization of phospholipase D from cabbage (1993), Biochim. Biophys. Acta, 1158, 1-7.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ba2+ | complete inhibition | Glycine max |  |
| 3.1.4.4 | Cu2+ | complete inhibition | Glycine max | |
| 3.1.4.4 | dithiobis-(2-nitrobenzoic acid) | - |
Glycine max | |
| 3.1.4.4 | EDTA | - |
Glycine max | |
| 3.1.4.4 | Mg2+ | - |
Glycine max | |
| 3.1.4.4 | Mn2+ | - |
Glycine max | |
| 3.1.4.4 | p-chloromercuribenzoate | complete inhibition | Glycine max | |
| 3.1.4.4 | Sr2+ | - |
Glycine max | |
| 3.1.4.4 | Zn2+ | - |
Glycine max | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.4 | 1.6 | - |
phosphatidylcholine | - |
Glycine max | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.4 | cytoplasm | - |
Glycine max | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | 20-100 mM required for maximal activity in plants | Glycine max | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 92000 | - |
gel filtration, SDS-PAGE | Glycine max |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phospholipid + H2O | Glycine max | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Glycine max | - |
soybean | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | to homogeneity | Glycine max |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | cell culture | - |
Glycine max | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Glycine max | 1,2-diacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phospholipid + H2O | phosphoric ester hydrolysis | Glycine max | phosphatidic acid + alcohol | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 5.5 | 7.5 | Ca2+ dependent | Glycine max |
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