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Literature summary extracted from

  • Abousalham, A.; Teissere, M.; Gardies, A.M.; Verger, R.; Noat, G.
    Phospholipase D from soybean (Glycine max L.) suspension-cultured cells: purification, structural and enzymatic properties (1995), Plant Cell Physiol., 36, 989-996.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ 20-100 mM required for maximal activity in plants Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 90000 92000 gel filtration, SDS-PAGE Brassica oleracea
3.1.4.4 90000 92000 calculated from amino acid analysis Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + H2O Brassica oleracea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Brassica oleracea
-
 savoy cabbage
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 complete to homogeneity Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 1492
-
-
 Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Brassica oleracea 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Brassica oleracea phosphatidic acid + alcohol
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 5.5 8 Ca2+ dependent Brassica oleracea