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Literature summary extracted from

  • Tzur, R.; Shapiro, B.
    Purification of phospholipase D from peanuts (1972), Biochim. Biophys. Acta, 280, 290-296.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 ethyl ether
-
 Arachis hypogaea
3.1.4.4 phosphatidic acid only with addition of lecithin and SDS Arachis hypogaea
3.1.4.4 SDS
-
 Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 albumin
-
 Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 2.2
-
 phosphatidylcholine
-
 Arachis hypogaea
3.1.4.4 2.5
-
 phosphatidylcholine transphosphaditylation in presence of methanol Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ 20-100 mM required for maximal activity in plants Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + alcohol Arachis hypogaea transphosphaditylation phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O Arachis hypogaea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Arachis hypogaea
-
 peanut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 partial Arachis hypogaea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 234
-
-
 Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
3.1.4.4 -20°C, lyophilyzed, 2 month, no loss of activity Arachis hypogaea
3.1.4.4 4°C, neutral pH, 50 days, no loss of activity Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + ethanolamine
-
 Arachis hypogaea phosphatidylethanolamine + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Arachis hypogaea 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + methanol
-
 Arachis hypogaea phosphatidylmethanol + choline
-
 ?
3.1.4.4 phospholipid + alcohol transphosphaditylation Arachis hypogaea phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Arachis hypogaea phosphatidic acid + alcohol
-
 ?