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Literature summary extracted from
- Studies on phospholipases from Streptomyces (1974), J. Biochem., 78, 363-372.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | ethyl ether | - |
Streptomyces hachijoensis |  |
| 3.1.4.4 | Triton X-100 | - |
Streptomyces hachijoensis | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.4 | 95% activity after 24 h at 25°C at neutral pH | Streptomyces hachijoensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Al3+ | 20% inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | Cd2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Cetylpyridinium chloride | - |
Streptomyces hachijoensis | |
| 3.1.4.4 | Co2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Cu2+ | slight inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | EDTA | 50% inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | Fe2+ | 30% inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | Fe3+ | 30% inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | Mg2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Mn2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | SDS | - |
Streptomyces hachijoensis | |
| 3.1.4.4 | Sn2+ | slight inhibition | Streptomyces hachijoensis | |
| 3.1.4.4 | sodium cholate | - |
Streptomyces hachijoensis | |
| 3.1.4.4 | sodium deoxycholate | - |
Streptomyces hachijoensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.4 | 4 | - |
phosphatidylethanolamine | - |
Streptomyces hachijoensis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Cd2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Co2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Mg2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Mn2+ | slight stimulation | Streptomyces hachijoensis | |
| 3.1.4.4 | Sn2+ | slight inhibition | Streptomyces hachijoensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 16000 | - |
gel filtration, SDS-PAGE | Streptomyces hachijoensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phospholipid + H2O | Streptomyces hachijoensis | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Streptomyces hachijoensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | to homogeneity | Streptomyces hachijoensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | culture supernatant | - |
Streptomyces hachijoensis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | cardiolipin + H2O | - |
Streptomyces hachijoensis | sn-glycero-1-phosphate + diacylglycerophosphate | - |
? | |
| 3.1.4.4 | lysophosphatidylcholine + H2O | - |
Streptomyces hachijoensis | monoacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces hachijoensis | 1,2-diacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylethanolamine + H2O | - |
Streptomyces hachijoensis | 1,2-diacylglycerophosphate + ethanolamine | - |
? | |
| 3.1.4.4 | phosphatidylserine + H2O | - |
Streptomyces hachijoensis | 1,2-diacylglycerophosphate + serine | - |
? | |
| 3.1.4.4 | phospholipid + H2O | phosphoric ester hydrolysis | Streptomyces hachijoensis | phosphatidic acid + alcohol | - |
? | |
| 3.1.4.4 | sphingomyelin + H2O | - |
Streptomyces hachijoensis | N-acylsphingosylphosphate + choline | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 50 | - |
- |
Streptomyces hachijoensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 50 | - |
stable for 90 min | Streptomyces hachijoensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 7.5 | - |
- |
Streptomyces hachijoensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 5 | 8 | - |
Streptomyces hachijoensis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 6 | 8 | 80% loss of activity after 24 h at 25°C at pH 4 | Streptomyces hachijoensis |
| 3.1.4.4 | 6 | 8 | 95% activity after 24 h at 25°C | Streptomyces hachijoensis |
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Release 2023.1 (January 2023)
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