BRENDA - Enzyme Database

Studies on phospholipases from Streptomyces

Okawa, Y.; Yamaguchi, T.; J. Biochem. 78, 363-372 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
-
Streptomyces hachijoensis
3.1.4.4
Triton X-100
-
Streptomyces hachijoensis
General Stability
EC Number
General Stability
Organism
3.1.4.4
95% activity after 24 h at 25C at neutral pH
Streptomyces hachijoensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Al3+
20% inhibition
Streptomyces hachijoensis
3.1.4.4
Cd2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cetylpyridinium chloride
-
Streptomyces hachijoensis
3.1.4.4
Co2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cu2+
slight inhibition
Streptomyces hachijoensis
3.1.4.4
EDTA
50% inhibition
Streptomyces hachijoensis
3.1.4.4
Fe2+
30% inhibition
Streptomyces hachijoensis
3.1.4.4
Fe3+
30% inhibition
Streptomyces hachijoensis
3.1.4.4
Mg2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mn2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
SDS
-
Streptomyces hachijoensis
3.1.4.4
Sn2+
slight inhibition
Streptomyces hachijoensis
3.1.4.4
sodium cholate
-
Streptomyces hachijoensis
3.1.4.4
sodium deoxycholate
-
Streptomyces hachijoensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
4
-
phosphatidylethanolamine
-
Streptomyces hachijoensis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cd2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Co2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mg2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mn2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Sn2+
slight inhibition
Streptomyces hachijoensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
16000
-
gel filtration, SDS-PAGE
Streptomyces hachijoensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Streptomyces hachijoensis
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Streptomyces hachijoensis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Streptomyces hachijoensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces hachijoensis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
cardiolipin + H2O
-
135435
Streptomyces hachijoensis
sn-glycero-1-phosphate + diacylglycerophosphate
-
-
-
?
3.1.4.4
lysophosphatidylcholine + H2O
-
135435
Streptomyces hachijoensis
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135435
Streptomyces hachijoensis
phosphatidic acid + alcohol
-
-
-
?
3.1.4.4
sphingomyelin + H2O
-
135435
Streptomyces hachijoensis
N-acylsphingosylphosphate + choline
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.4
50
-
-
Streptomyces hachijoensis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.4
50
-
stable for 90 min
Streptomyces hachijoensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
7.5
-
-
Streptomyces hachijoensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
8
-
Streptomyces hachijoensis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
6
8
80% loss of activity after 24 h at 25C at pH 4; 95% activity after 24 h at 25C
Streptomyces hachijoensis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
-
Streptomyces hachijoensis
3.1.4.4
Triton X-100
-
Streptomyces hachijoensis
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.4
95% activity after 24 h at 25C at neutral pH
Streptomyces hachijoensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Al3+
20% inhibition
Streptomyces hachijoensis
3.1.4.4
Cd2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cetylpyridinium chloride
-
Streptomyces hachijoensis
3.1.4.4
Co2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cu2+
slight inhibition
Streptomyces hachijoensis
3.1.4.4
EDTA
50% inhibition
Streptomyces hachijoensis
3.1.4.4
Fe2+
30% inhibition
Streptomyces hachijoensis
3.1.4.4
Fe3+
30% inhibition
Streptomyces hachijoensis
3.1.4.4
Mg2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mn2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
SDS
-
Streptomyces hachijoensis
3.1.4.4
Sn2+
slight inhibition
Streptomyces hachijoensis
3.1.4.4
sodium cholate
-
Streptomyces hachijoensis
3.1.4.4
sodium deoxycholate
-
Streptomyces hachijoensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
4
-
phosphatidylethanolamine
-
Streptomyces hachijoensis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Cd2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Co2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mg2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Mn2+
slight stimulation
Streptomyces hachijoensis
3.1.4.4
Sn2+
slight inhibition
Streptomyces hachijoensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
16000
-
gel filtration, SDS-PAGE
Streptomyces hachijoensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Streptomyces hachijoensis
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Streptomyces hachijoensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Streptomyces hachijoensis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
cardiolipin + H2O
-
135435
Streptomyces hachijoensis
sn-glycero-1-phosphate + diacylglycerophosphate
-
-
-
?
3.1.4.4
lysophosphatidylcholine + H2O
-
135435
Streptomyces hachijoensis
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135435
Streptomyces hachijoensis
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135435
Streptomyces hachijoensis
phosphatidic acid + alcohol
-
-
-
?
3.1.4.4
sphingomyelin + H2O
-
135435
Streptomyces hachijoensis
N-acylsphingosylphosphate + choline
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.4
50
-
-
Streptomyces hachijoensis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.4
50
-
stable for 90 min
Streptomyces hachijoensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
7.5
-
-
Streptomyces hachijoensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
8
-
Streptomyces hachijoensis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
6
8
80% loss of activity after 24 h at 25C at pH 4; 95% activity after 24 h at 25C
Streptomyces hachijoensis