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Literature summary extracted from

  • Imamura, S.; Horiuti, Y.
    Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose (1979), J. Biochem., 85, 79-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 albumin
-
 Streptomyces chromofuscus
3.1.4.4 ethyl ether
-
 Streptomyces chromofuscus
3.1.4.4 sodium deoxycholate
-
 Streptomyces chromofuscus
3.1.4.4 Triton X-100
-
 Streptomyces chromofuscus

General Stability

EC Number General Stability Organism
3.1.4.4 albumin increases stability of the enzyme Streptomyces chromofuscus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 Ba2+ complete inhibition Streptomyces chromofuscus
3.1.4.4 Co2+ complete inhibition Streptomyces chromofuscus
3.1.4.4 EDTA
-
 Streptomyces chromofuscus
3.1.4.4 Mg2+ stimulates in the absence of Triton X-100 Streptomyces chromofuscus
3.1.4.4 Mn2+
-
 Streptomyces chromofuscus
3.1.4.4 SDS
-
 Streptomyces chromofuscus
3.1.4.4 Sn2+ complete inhibition Streptomyces chromofuscus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.56
-
 sphingomyelin
-
 Streptomyces chromofuscus
3.1.4.4 1.43
-
 phosphatidylcholine
-
 Streptomyces chromofuscus
3.1.4.4 1.67
-
 lysophosphatidylcholine
-
 Streptomyces chromofuscus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+
-
 Streptomyces chromofuscus
3.1.4.4 Mg2+ stimulates in the absence of Triton X-100 Streptomyces chromofuscus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 50000 57000 gel filtration, SDS-PAGE Streptomyces chromofuscus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + H2O Streptomyces chromofuscus phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces chromofuscus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 to homogeneity Streptomyces chromofuscus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 culture supernatant
-
 Streptomyces chromofuscus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 lysophosphatidylcholine + H2O
-
 Streptomyces chromofuscus monoacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces chromofuscus 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylserine + H2O
-
 Streptomyces chromofuscus 1,2-diacylglycerophosphate + serine
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Streptomyces chromofuscus phosphatidic acid + alcohol
-
 ?
3.1.4.4 sphingomyelin + H2O
-
 Streptomyces chromofuscus N-acylsphingosylphosphate + choline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 70
-
 50% acivity Streptomyces chromofuscus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 8
-
-
 Streptomyces chromofuscus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 5 8
-
 Streptomyces chromofuscus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 10
-
 best stability Streptomyces chromofuscus