BRENDA - Enzyme Database

Partial purification and properties of a rat brain phospholipase

Taki, T.; Kanfer, J.N.; J. Biol. Chem. 254, 9761-9765 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
stimulates in the presence of Ca2+
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
EDTA
slight inhibition
Rattus norvegicus
3.1.4.4
Fe2+
-
Rattus norvegicus
3.1.4.4
Fe3+
complete inhibition at 5 mM
Rattus norvegicus
3.1.4.4
Mg2+
-
Rattus norvegicus
3.1.4.4
Mn2+
-
Rattus norvegicus
3.1.4.4
p-chloromercuribenzoate
complete inhibition
Rattus norvegicus
3.1.4.4
SDS
-
Rattus norvegicus
3.1.4.4
sodium taurocholate
-
Rattus norvegicus
3.1.4.4
Triton CF-54
-
Rattus norvegicus
3.1.4.4
Triton CF-54/Tween 80
-
Rattus norvegicus
3.1.4.4
Triton X-100
-
Rattus norvegicus
3.1.4.4
Tween 20
-
Rattus norvegicus
3.1.4.4
Tween 80
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.75
-
phosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.78
-
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.91
-
phosphatidylethanolamine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
not required for activity
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
200000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Rattus norvegicus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dipalmitoylphosphatidylcholine + H2O
-
135433
Rattus norvegicus
dipalmitoylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135433
Rattus norvegicus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135433
Rattus norvegicus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
-
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135433
Rattus norvegicus
phosphatidic acid + alcohol
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.4
60
80
70% loss of activity after 3 min at 60C; complete loss of activity after 3 min at 80C
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
8
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
stimulates in the presence of Ca2+
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
EDTA
slight inhibition
Rattus norvegicus
3.1.4.4
Fe2+
-
Rattus norvegicus
3.1.4.4
Fe3+
complete inhibition at 5 mM
Rattus norvegicus
3.1.4.4
Mg2+
-
Rattus norvegicus
3.1.4.4
Mn2+
-
Rattus norvegicus
3.1.4.4
p-chloromercuribenzoate
complete inhibition
Rattus norvegicus
3.1.4.4
SDS
-
Rattus norvegicus
3.1.4.4
sodium taurocholate
-
Rattus norvegicus
3.1.4.4
Triton CF-54
-
Rattus norvegicus
3.1.4.4
Triton CF-54/Tween 80
-
Rattus norvegicus
3.1.4.4
Triton X-100
-
Rattus norvegicus
3.1.4.4
Tween 20
-
Rattus norvegicus
3.1.4.4
Tween 80
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.75
-
phosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.78
-
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.91
-
phosphatidylethanolamine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
not required for activity
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
200000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Rattus norvegicus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dipalmitoylphosphatidylcholine + H2O
-
135433
Rattus norvegicus
dipalmitoylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135433
Rattus norvegicus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135433
Rattus norvegicus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
-
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135433
Rattus norvegicus
phosphatidic acid + alcohol
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.4
60
80
70% loss of activity after 3 min at 60C; complete loss of activity after 3 min at 80C
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
8
-
Rattus norvegicus