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Literature summary extracted from

  • Taki, T.; Kanfer, J.N.
    Partial purification and properties of a rat brain phospholipase (1979), J. Biol. Chem., 254, 9761-9765.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 ethyl ether stimulates in the presence of Ca2+ Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 EDTA slight inhibition Rattus norvegicus
3.1.4.4 Fe2+
-
 Rattus norvegicus
3.1.4.4 Fe3+ complete inhibition at 5 mM Rattus norvegicus
3.1.4.4 Mg2+
-
 Rattus norvegicus
3.1.4.4 Mn2+
-
 Rattus norvegicus
3.1.4.4 p-chloromercuribenzoate complete inhibition Rattus norvegicus
3.1.4.4 SDS
-
 Rattus norvegicus
3.1.4.4 sodium taurocholate
-
 Rattus norvegicus
3.1.4.4 Triton CF-54
-
 Rattus norvegicus
3.1.4.4 Triton CF-54/Tween 80
-
 Rattus norvegicus
3.1.4.4 Triton X-100
-
 Rattus norvegicus
3.1.4.4 Tween 20
-
 Rattus norvegicus
3.1.4.4 Tween 80
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.75
-
 phosphatidylcholine
-
 Rattus norvegicus
3.1.4.4 0.78
-
 dipalmitoylphosphatidylcholine
-
 Rattus norvegicus
3.1.4.4 0.91
-
 phosphatidylethanolamine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ not required for activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 200000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + H2O Rattus norvegicus phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 dipalmitoylphosphatidylcholine + H2O
-
 Rattus norvegicus dipalmitoylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Rattus norvegicus 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Rattus norvegicus 1,2-diacylglycerophosphate + ethanolamine
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Rattus norvegicus phosphatidic acid + alcohol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 60 80 70% loss of activity after 3 min at 60°C Rattus norvegicus
3.1.4.4 60 80 complete loss of activity after 3 min at 80°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 6
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 5 8
-
 Rattus norvegicus