BRENDA - Enzyme Database

Phospholipase D from rat brain

Taki, T.; Kanfer, J.N.; Methods Enzymol. 71, 746-750 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Fe2+
-
Rattus norvegicus
3.1.4.4
Fe3+
complete inhibition at 5 mM
Rattus norvegicus
3.1.4.4
p-chloromercuribenzoate
-
Rattus norvegicus
3.1.4.4
SDS
-
Rattus norvegicus
3.1.4.4
Triton X-100
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.75
-
phosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.78
-
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.91
-
phosphatidylethanolamine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Rattus norvegicus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
0.002
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dipalmitoylphosphatidylcholine + H2O
-
135429
Rattus norvegicus
dipalmitoylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135429
Rattus norvegicus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135429
Rattus norvegicus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135429
Rattus norvegicus
phosphatidic acid + alcohol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Fe2+
-
Rattus norvegicus
3.1.4.4
Fe3+
complete inhibition at 5 mM
Rattus norvegicus
3.1.4.4
p-chloromercuribenzoate
-
Rattus norvegicus
3.1.4.4
SDS
-
Rattus norvegicus
3.1.4.4
Triton X-100
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.75
-
phosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.78
-
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
3.1.4.4
0.91
-
phosphatidylethanolamine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + H2O
Rattus norvegicus
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
0.002
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dipalmitoylphosphatidylcholine + H2O
-
135429
Rattus norvegicus
dipalmitoylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135429
Rattus norvegicus
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135429
Rattus norvegicus
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135429
Rattus norvegicus
phosphatidic acid + alcohol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6
-
-
Rattus norvegicus