BRENDA - Enzyme Database

Purification and properties of phospholipase D from Actinomadura sp.strain no.362

Kokusho, Y.; Kato, S.; Machida, H.; Iwasaki, S.; Agric. Biol. Chem. 51, 2515-2524 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
cholic acid
-
Actinomadura sp.
3.1.4.4
ethyl ether
stimulates
Actinomadura sp.
3.1.4.4
sodium deoxycholate
-
Actinomadura sp.
3.1.4.4
Triton X-100
substrate-dependent activation
Actinomadura sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Ag2+
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
Al3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Cetylpyridinium chloride
-
Actinomadura sp.
3.1.4.4
cetyltrimethylammonium bromide
-
Actinomadura sp.
3.1.4.4
Fe2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Fe3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Hg2+
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
Mn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
SDS
-
Actinomadura sp.
3.1.4.4
Sn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Triton X-100
substrate-dependent inhibition
Actinomadura sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.7
-
phosphatidylcholine
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
1.07
-
lysophosphatidylcholine
in the presence of Triton X-100
Actinomadura sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
extracellular
-
Actinomadura sp.
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Al3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Ca2+
-
Actinomadura sp.
3.1.4.4
Fe2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Fe3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Mn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Sn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
50000
60000
gel filtration, SDS-PAGE
Actinomadura sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Actinomadura sp.
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Actinomadura sp.
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Actinomadura sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Actinomadura sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Actinomadura sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
688
-
-
Actinomadura sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + H2O
-
135428
Actinomadura sp.
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
-
135428
Actinomadura sp.
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135428
Actinomadura sp.
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135428
Actinomadura sp.
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135428
Actinomadura sp.
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135428
Actinomadura sp.
phosphatidic acid + alcohol
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
50
-
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
60
70
in the absence of Triton X-100
Actinomadura sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
30
-
at pH 7.2 for 30 min; irreversible loss of activity at higher temperature
Actinomadura sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
7
Triton X-100 dependent
Actinomadura sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
4
8
2 h at 25°C
Actinomadura sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
cholic acid
-
Actinomadura sp.
3.1.4.4
ethyl ether
stimulates
Actinomadura sp.
3.1.4.4
sodium deoxycholate
-
Actinomadura sp.
3.1.4.4
Triton X-100
substrate-dependent activation
Actinomadura sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Ag2+
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
Al3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Cetylpyridinium chloride
-
Actinomadura sp.
3.1.4.4
cetyltrimethylammonium bromide
-
Actinomadura sp.
3.1.4.4
Fe2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Fe3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Hg2+
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
Mn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
SDS
-
Actinomadura sp.
3.1.4.4
Sn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Triton X-100
substrate-dependent inhibition
Actinomadura sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.7
-
phosphatidylcholine
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
1.07
-
lysophosphatidylcholine
in the presence of Triton X-100
Actinomadura sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
extracellular
-
Actinomadura sp.
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Al3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Ca2+
-
Actinomadura sp.
3.1.4.4
Fe2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Fe3+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Mn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
3.1.4.4
Sn2+
stimulates in the absence of Triton X-100
Actinomadura sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
50000
60000
gel filtration, SDS-PAGE
Actinomadura sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Actinomadura sp.
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Actinomadura sp.
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
to homogeneity
Actinomadura sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
culture supernatant
-
Actinomadura sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
688
-
-
Actinomadura sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + H2O
-
135428
Actinomadura sp.
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
-
135428
Actinomadura sp.
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135428
Actinomadura sp.
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135428
Actinomadura sp.
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135428
Actinomadura sp.
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135428
Actinomadura sp.
phosphatidic acid + alcohol
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
50
-
in the presence of Triton X-100
Actinomadura sp.
3.1.4.4
60
70
in the absence of Triton X-100
Actinomadura sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
30
-
at pH 7.2 for 30 min; irreversible loss of activity at higher temperature
Actinomadura sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
7
Triton X-100 dependent
Actinomadura sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
4
8
2 h at 25°C
Actinomadura sp.