BRENDA - Enzyme Database

Phospholipase D from savoy cabbage

Yang, S.F.; Methods Enzymol. 14, 208-210 (1969)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
essential
Brassica oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
p-chloromercuribenzoate
complete inhibition
Brassica oleracea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Brassica oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Brassica oleracea
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Brassica oleracea
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Brassica oleracea
-
savoy cabbage
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
partial
Brassica oleracea
Reaction
EC Number
Reaction
Commentary
Organism
3.1.4.4
a phosphatidylcholine + H2O = choline + a phosphatidate
acts also on other phosphatidyl esters
Brassica oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
leaf
-
Brassica oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
1.1
-
-
Brassica oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dihexanoylphosphatidylcholine + H2O
-
135426
Brassica oleracea
dihexanoylglycerophosphate + choline
-
-
-
?
3.1.4.4
lysophosphatidylcholine + H2O
-
135426
Brassica oleracea
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
transferase activity in presence of 4% ethanolamine
135426
Brassica oleracea
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
135426
Brassica oleracea
sn-glycero-1-phosphate + diacylglycerophosphate
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135426
Brassica oleracea
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135426
Brassica oleracea
phosphatidic acid + alcohol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.6
-
-
Brassica oleracea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
6.5
-
Brassica oleracea
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
6.5
7
-
Brassica oleracea
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
essential
Brassica oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
p-chloromercuribenzoate
complete inhibition
Brassica oleracea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Brassica oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Brassica oleracea
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Brassica oleracea
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
partial
Brassica oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
leaf
-
Brassica oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
1.1
-
-
Brassica oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
dihexanoylphosphatidylcholine + H2O
-
135426
Brassica oleracea
dihexanoylglycerophosphate + choline
-
-
-
?
3.1.4.4
lysophosphatidylcholine + H2O
-
135426
Brassica oleracea
monoacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
transferase activity in presence of 4% ethanolamine
135426
Brassica oleracea
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
135426
Brassica oleracea
sn-glycero-1-phosphate + diacylglycerophosphate
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
135426
Brassica oleracea
1,2-diacylglycerophosphate + serine
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135426
Brassica oleracea
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135426
Brassica oleracea
phosphatidic acid + alcohol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.6
-
-
Brassica oleracea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.4
5
6.5
-
Brassica oleracea
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.4
6.5
7
-
Brassica oleracea