BRENDA - Enzyme Database

Crystal structure of phospholipase C from Bacillus cereus complexed with a substrate analogue

Hansen, S.; Hough, E.; Svensson, L.A.; Wong, Y.L.; Martin, S.F.; J. Mol. Biol. 234, 179-189 (1993)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.4.3
1.9 A resolution, R-factor of 15.7%; crystal structure of a complex between the enzyme and a competitive inhibitor. 1.9 A resolution, R-factor of 15.7%
Bacillus cereus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
3(S),4-dihexanoylbutyl-1-phosphonycholine
non-hydrolyzable, competitive inhibitor of bacterial PLC, analog of the natural phospholipid substrate, binds with its phosphonyl group to the three Zn-ions in the active site of the enzyme
Bacillus cereus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.38
-
phosphatidylcholine
water-soluble substrate
Bacillus cereus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
3 Zn2+ ions in active site; in the active site of the enzyme; metalloenzyme
Bacillus cereus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Bacillus cereus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
-
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
-
135311
Bacillus cereus
1,2-diacylglycerol + choline phosphate
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135311
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
-
-
?
3.1.4.3
phosphatidylserine + H2O
-
135311
Bacillus cereus
phosphorylserine + diacylglycerol
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.3
0.47
-
3(S),4-dihexanoylbutyl-1-phosphonycholine
-
Bacillus cereus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.4.3
1.9 A resolution, R-factor of 15.7%; crystal structure of a complex between the enzyme and a competitive inhibitor. 1.9 A resolution, R-factor of 15.7%
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
3(S),4-dihexanoylbutyl-1-phosphonycholine
non-hydrolyzable, competitive inhibitor of bacterial PLC, analog of the natural phospholipid substrate, binds with its phosphonyl group to the three Zn-ions in the active site of the enzyme
Bacillus cereus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.3
0.47
-
3(S),4-dihexanoylbutyl-1-phosphonycholine
-
Bacillus cereus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.38
-
phosphatidylcholine
water-soluble substrate
Bacillus cereus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
3 Zn2+ ions in active site; in the active site of the enzyme; metalloenzyme
Bacillus cereus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
-
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
-
135311
Bacillus cereus
1,2-diacylglycerol + choline phosphate
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135311
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
-
-
?
3.1.4.3
phosphatidylserine + H2O
-
135311
Bacillus cereus
phosphorylserine + diacylglycerol
-
-
-
?