BRENDA - Enzyme Database

Phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus

Zwaal, R.F.A.; Roelofsen, B.; Methods Enzymol. 32, 154-161 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
substrate: egg yolk lipoprotein
Bacillus cereus
General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate
Bacillus cereus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
dithiothreitol
-
Bacillus cereus
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
o-phenanthroline
-
Bacillus cereus
3.1.4.3
SDS
-
Bacillus cereus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mn2+
restores activity after EDTA treatment
Bacillus cereus
3.1.4.3
Zn2+
restores activity after EDTA treatment
Bacillus cereus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
gel filtration
Bacillus cereus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Bacillus cereus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure
Bacillus cereus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
1010
-
substrate: lecithin
Bacillus cereus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, 50% glycerol, 6 months, no loss of activity
Bacillus cereus
3.1.4.3
20°C, 50% glycerol, 1 week, no significant loss of activity
Bacillus cereus
3.1.4.3
20°C, 50% glycerol, after 1 week: 1% loss of activity per day
Bacillus cereus
3.1.4.3
4°C, impure preparations, 77% saturated with (NH4)2SO4, stable
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
cardiolipin + H2O
-
135308
Bacillus cereus
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
glucosaminylphosphatidylglycerol + H2O
-
135308
Bacillus cereus
?
-
-
-
?
3.1.4.3
phosphatidic acid + H2O
-
135308
Bacillus cereus
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
135308
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135308
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylglycerol + H2O
-
135308
Bacillus cereus
1,2-diacylglycerol + phosphate
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylinositol + H2O
-
135308
Bacillus cereus
inositol monophosphate + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135308
Bacillus cereus
phosphorylserine + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
135308
Bacillus cereus
ceramide + phosphorylcholine
-
135308
Bacillus cereus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
70
-
for 20 min, no significant loss of activity
Bacillus cereus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
substrate: egg yolk lipoprotein
Bacillus cereus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
dithiothreitol
-
Bacillus cereus
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
o-phenanthroline
-
Bacillus cereus
3.1.4.3
SDS
-
Bacillus cereus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mn2+
restores activity after EDTA treatment
Bacillus cereus
3.1.4.3
Zn2+
restores activity after EDTA treatment
Bacillus cereus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
gel filtration
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure
Bacillus cereus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
1010
-
substrate: lecithin
Bacillus cereus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, 50% glycerol, 6 months, no loss of activity
Bacillus cereus
3.1.4.3
20°C, 50% glycerol, 1 week, no significant loss of activity
Bacillus cereus
3.1.4.3
20°C, 50% glycerol, after 1 week: 1% loss of activity per day
Bacillus cereus
3.1.4.3
4°C, impure preparations, 77% saturated with (NH4)2SO4, stable
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
cardiolipin + H2O
-
135308
Bacillus cereus
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
glucosaminylphosphatidylglycerol + H2O
-
135308
Bacillus cereus
?
-
-
-
?
3.1.4.3
phosphatidic acid + H2O
-
135308
Bacillus cereus
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
135308
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135308
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylglycerol + H2O
-
135308
Bacillus cereus
1,2-diacylglycerol + phosphate
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylinositol + H2O
-
135308
Bacillus cereus
inositol monophosphate + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135308
Bacillus cereus
phosphorylserine + diacylglycerol
-
135308
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
135308
Bacillus cereus
ceramide + phosphorylcholine
-
135308
Bacillus cereus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
70
-
for 20 min, no significant loss of activity
Bacillus cereus