BRENDA - Enzyme Database

Studies on phospholipase C from Pseudomonas aureofaciens. Purification and some properties of phospholipase C

Sonoki, S.; Ikezawa, H.; Biochim. Biophys. Acta 403, 412-424 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
-
Pseudomonas chlororaphis subsp. aureofaciens
General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Pseudomonas chlororaphis subsp. aureofaciens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Ca2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
cetylmethylammonium bromide
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Cu2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
EDTA
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Fe2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mg2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
o-phenanthroline
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Tris buffer
in alkaline range
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Zn2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas chlororaphis subsp. aureofaciens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mg2+
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
-
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
35000
-
gel filtration
Pseudomonas chlororaphis subsp. aureofaciens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Pseudomonas chlororaphis subsp. aureofaciens
-
1,2-diacylglycerol + phosphorylcholine
-
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
-
1,2-diacylglycerol + phosphorylcholine
-
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
3.1.4.3
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
gel filtration, ion-exchange chromatography, 3645fold, apparently pure
Pseudomonas chlororaphis subsp. aureofaciens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
17.5
-
substrate: phosphatidylcholine
Pseudomonas chlororaphis subsp. aureofaciens
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, in Na2B4O7/HCl buffer, pH 7.5, 40% glycerol, no loss of activity after 6 months
Pseudomonas chlororaphis subsp. aureofaciens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
lysophosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
monoacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
monoacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
3.1.4.3
phosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
1,2-diacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
1,2-diacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
phosphorylethanolamine + diacylglycerol
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
phosphorylethanolamine + diacylglycerol
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
70
-
for 10 min, deactivation
Pseudomonas chlororaphis subsp. aureofaciens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.5
8
substrate: phosphatidylcholine
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
8
8.5
substrate: phosphatidylethanolamine
Pseudomonas chlororaphis subsp. aureofaciens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
6.5
9
-
Pseudomonas chlororaphis subsp. aureofaciens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
-
Pseudomonas chlororaphis subsp. aureofaciens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Pseudomonas chlororaphis subsp. aureofaciens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Ca2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
cetylmethylammonium bromide
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Cu2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
EDTA
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Fe2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mg2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
inhibits phosphatidylethanolamine hydrolysis
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
o-phenanthroline
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Tris buffer
in alkaline range
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Zn2+
4 mM
Pseudomonas chlororaphis subsp. aureofaciens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas chlororaphis subsp. aureofaciens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mg2+
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
-
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
35000
-
gel filtration
Pseudomonas chlororaphis subsp. aureofaciens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Pseudomonas chlororaphis subsp. aureofaciens
-
1,2-diacylglycerol + phosphorylcholine
-
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
-
1,2-diacylglycerol + phosphorylcholine
-
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
gel filtration, ion-exchange chromatography, 3645fold, apparently pure
Pseudomonas chlororaphis subsp. aureofaciens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
17.5
-
substrate: phosphatidylcholine
Pseudomonas chlororaphis subsp. aureofaciens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, in Na2B4O7/HCl buffer, pH 7.5, 40% glycerol, no loss of activity after 6 months
Pseudomonas chlororaphis subsp. aureofaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
lysophosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
monoacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
monoacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
3.1.4.3
phosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
1,2-diacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
1,2-diacylglycerol + phosphorylcholine
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
phosphorylethanolamine + diacylglycerol
-
135306
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
phosphorylethanolamine + diacylglycerol
-
135306
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
70
-
for 10 min, deactivation
Pseudomonas chlororaphis subsp. aureofaciens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.5
8
substrate: phosphatidylcholine
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
8
8.5
substrate: phosphatidylethanolamine
Pseudomonas chlororaphis subsp. aureofaciens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
6.5
9
-
Pseudomonas chlororaphis subsp. aureofaciens