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Literature summary extracted from
- Studies on phospholipase C from Pseudomonas aureofaciens. Purification and some properties of phospholipase C (1975), Biochim. Biophys. Acta, 403, 412-424.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | sodium deoxycholate | - |
Pseudomonas chlororaphis subsp. aureofaciens |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.3 | 1,10-phenanthroline, denaturation | Pseudomonas chlororaphis subsp. aureofaciens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | Ca2+ | inhibits phosphatidylethanolamine hydrolysis | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | cetylmethylammonium bromide | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Cu2+ | 4 mM | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | EDTA | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Fe2+ | 4 mM | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Mg2+ | inhibits phosphatidylethanolamine hydrolysis | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Mn2+ | inhibits phosphatidylethanolamine hydrolysis | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | o-phenanthroline | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Tris buffer | in alkaline range | Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Zn2+ | 4 mM | Pseudomonas chlororaphis subsp. aureofaciens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.3 | extracellular | - |
Pseudomonas chlororaphis subsp. aureofaciens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | Ca2+ | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Mg2+ | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.1.4.3 | Mn2+ | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 35000 | - |
gel filtration | Pseudomonas chlororaphis subsp. aureofaciens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | phosphatidylcholine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.3 | Pseudomonas chlororaphis subsp. aureofaciens | - |
- |
- |
| 3.1.4.3 | Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.3 | gel filtration, ion-exchange chromatography, 3645fold, apparently pure | Pseudomonas chlororaphis subsp. aureofaciens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 17.5 | - |
substrate: phosphatidylcholine | Pseudomonas chlororaphis subsp. aureofaciens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.3 | -20°C, in Na2B4O7/HCl buffer, pH 7.5, 40% glycerol, no loss of activity after 6 months | Pseudomonas chlororaphis subsp. aureofaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | lysophosphatidylcholine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | monoacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | lysophosphatidylcholine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 | monoacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylethanolamine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | phosphorylethanolamine + diacylglycerol | - |
? | |
| 3.1.4.3 | phosphatidylethanolamine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 | phosphorylethanolamine + diacylglycerol | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 70 | - |
for 10 min, deactivation | Pseudomonas chlororaphis subsp. aureofaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 7.5 | 8 | substrate: phosphatidylcholine | Pseudomonas chlororaphis subsp. aureofaciens |
| 3.1.4.3 | 8 | 8.5 | substrate: phosphatidylethanolamine | Pseudomonas chlororaphis subsp. aureofaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 6.5 | 9 | - |
Pseudomonas chlororaphis subsp. aureofaciens |
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