BRENDA - Enzyme Database

Studies on phospholipase C from Pseudomonas aureofaciens. Further studies on the properties of the enzyme

Sonoki, S.; Ikezawa, H.; J. Biochem. 80, 361-366 (1976)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Pseudomonas chlororaphis subsp. aureofaciens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
ethyl alcohol
at a concentration above 2%
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
o-phenanthroline
-
Pseudomonas chlororaphis subsp. aureofaciens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas chlororaphis subsp. aureofaciens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Co2+
restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Zn2+
metalloenzyme; restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
35000
-
-
Pseudomonas chlororaphis subsp. aureofaciens
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
ethyl alcohol
inhibitory at concentrations above 2%
Pseudomonas chlororaphis subsp. aureofaciens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
lysophosphatidylcholine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
monoacylglycerol + phosphorylcholine
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
1,2-diacylglycerol + phosphorylcholine
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
phosphorylethanolamine + diacylglycerol
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Pseudomonas chlororaphis subsp. aureofaciens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
-
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
ethyl alcohol
at a concentration above 2%
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
o-phenanthroline
-
Pseudomonas chlororaphis subsp. aureofaciens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas chlororaphis subsp. aureofaciens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Co2+
restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Mn2+
restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
3.1.4.3
Zn2+
metalloenzyme; restores activity after EDTA treatment; restores activity after o-phenanthroline-treatment
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
35000
-
-
Pseudomonas chlororaphis subsp. aureofaciens
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
ethyl alcohol
inhibitory at concentrations above 2%
Pseudomonas chlororaphis subsp. aureofaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
lysophosphatidylcholine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
monoacylglycerol + phosphorylcholine
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylcholine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
1,2-diacylglycerol + phosphorylcholine
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
phosphorylethanolamine + diacylglycerol
-
135305
Pseudomonas chlororaphis subsp. aureofaciens
?