BRENDA - Enzyme Database

Some characteristics of phospholipase C from Bacillus cereus

Otnaess, A.B.; Little, C.; Sletten, K.; Wallin, R.; Johnsen, S.; Flengsrud, R.; Prydz, H.; Eur. J. Biochem. 79, 459-468 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
0.1%
Bacillus cereus
Application
EC Number
Application
Commentary
Organism
3.1.4.3
medicine
inactivation of thromboplastin, potential therapeutic value in cases of intravascular coagulation
Bacillus cereus
General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
guanidine HCl, stable
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate, denaturation
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate, enzyme resistant to denaturation in presence of 1 mM Zn2+
Bacillus cereus
3.1.4.3
urea, enzyme stable in presence of 1 mM Zn2+
Bacillus cereus
3.1.4.3
urea, PLC is catalytically active in 8 M urea
Bacillus cereus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
o-phenanthroline
denaturation
Bacillus cereus
3.1.4.3
SDS
-
Bacillus cereus
3.1.4.3
Urea
denaturation; denaturation only in absence of free Zn2+
Bacillus cereus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.6
-
dipalmitoylphosphatidylcholine
-
Bacillus cereus
3.1.4.3
2
3
phosphatidylserine
-
Bacillus cereus
3.1.4.3
4.5
7
Dipalmitoylphosphatidylethanolamine
-
Bacillus cereus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
2 mol Zn2+ per mol enzyme; metalloenzyme
Bacillus cereus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
-
Bacillus cereus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Bacillus cereus
-
strain ATCC 10987 AB-1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
-
Bacillus cereus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, in barbital-buffered saline, pH 7.4, 1 mM ZnSO4, stable for more than one year
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dicaproylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dicaproylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dimyristoylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dimyristoylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dipalmitoylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dipalmitoylphosphatidylethanolamine + H2O
-
135304
Bacillus cereus
1,2-dipalmitoylglycerol + phosphorylethanolamine
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135304
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135304
Bacillus cereus
phosphorylserine + diacylglycerol
-
135304
Bacillus cereus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
23000, no comment on method
Bacillus cereus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.3
35
60
substrate: dimyristoylphosphatidylcholine
Bacillus cereus
3.1.4.3
47
-
substrate: dipalmitoylphosphatidylcholine
Bacillus cereus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.4.3
10
76
-
Bacillus cereus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
76
-
for 6 min, with 0.1 mM free Zn2+: loss of 21% of activity, without Zn2+: loss of 63% of activity
Bacillus cereus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
8.3
subatrate: dipalmitoylphosphatidylcholine
Bacillus cereus
3.1.4.3
8
-
substrate: dicaproyl phosphatidylcholine
Bacillus cereus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium deoxycholate
0.1%
Bacillus cereus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.4.3
medicine
inactivation of thromboplastin, potential therapeutic value in cases of intravascular coagulation
Bacillus cereus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
guanidine HCl, stable
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate, denaturation
Bacillus cereus
3.1.4.3
sodium dodecyl sulfate, enzyme resistant to denaturation in presence of 1 mM Zn2+
Bacillus cereus
3.1.4.3
urea, enzyme stable in presence of 1 mM Zn2+
Bacillus cereus
3.1.4.3
urea, PLC is catalytically active in 8 M urea
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
o-phenanthroline
denaturation
Bacillus cereus
3.1.4.3
SDS
-
Bacillus cereus
3.1.4.3
Urea
denaturation; denaturation only in absence of free Zn2+
Bacillus cereus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.6
-
dipalmitoylphosphatidylcholine
-
Bacillus cereus
3.1.4.3
2
3
phosphatidylserine
-
Bacillus cereus
3.1.4.3
4.5
7
Dipalmitoylphosphatidylethanolamine
-
Bacillus cereus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
2 mol Zn2+ per mol enzyme; metalloenzyme
Bacillus cereus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
-
Bacillus cereus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
-
Bacillus cereus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, in barbital-buffered saline, pH 7.4, 1 mM ZnSO4, stable for more than one year
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dicaproylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dicaproylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dimyristoylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dimyristoylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-dipalmitoylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
1,2-dipalmitoylphosphatidylethanolamine + H2O
-
135304
Bacillus cereus
1,2-dipalmitoylglycerol + phosphorylethanolamine
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
-
135304
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135304
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135304
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135304
Bacillus cereus
phosphorylserine + diacylglycerol
-
135304
Bacillus cereus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
23000, no comment on method
Bacillus cereus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.3
35
60
substrate: dimyristoylphosphatidylcholine
Bacillus cereus
3.1.4.3
47
-
substrate: dipalmitoylphosphatidylcholine
Bacillus cereus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.4.3
10
76
-
Bacillus cereus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
76
-
for 6 min, with 0.1 mM free Zn2+: loss of 21% of activity, without Zn2+: loss of 63% of activity
Bacillus cereus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
8.3
subatrate: dipalmitoylphosphatidylcholine
Bacillus cereus
3.1.4.3
8
-
substrate: dicaproyl phosphatidylcholine
Bacillus cereus