Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Little, C.
    Phospholipase C from Bacillus cereus (1981), Methods Enzymol., 71, 725-730.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.3
-
 Bacillus cereus

General Stability

EC Number General Stability Organism
3.1.4.3 1,10-phenanthroline, denaturation Bacillus cereus
3.1.4.3 urea, PLC is catalytically active in 8 M urea Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Cd2+ in absence of free Zn2+ in solution Bacillus cereus
3.1.4.3 Cu2+ in absence of free Zn2+ in solution Bacillus cereus
3.1.4.3 EDTA
-
 Bacillus cereus
3.1.4.3 Ni2+ in absence of free Zn2+ in solution Bacillus cereus
3.1.4.3 o-phenanthroline
-
 Bacillus cereus
3.1.4.3 Urea denaturation Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ metalloenzyme Bacillus cereus
3.1.4.3 Zn2+ 2 mol Zn2+ per mol enzyme Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 23000
-
 ultracentrifugation, SDS-PAGE, amino acid calculation Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Bacillus cereus
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 sphingomyelin + H2O Bacillus cereus
-
 ceramide + phosphorylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
 strain ATCC 10987 AB-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 affinity chromatography steps, column chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 1510
-
-
 Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C, 1-2 mg purified enzyme/ml, indefinitely stable Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Bacillus cereus phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylinositol + H2O
-
 Bacillus cereus inositol monophosphate + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Bacillus cereus phosphorylserine + diacylglycerol
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Bacillus cereus ceramide + phosphorylcholine
-
 ?
3.1.4.3 sphingomyelin + H2O when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath Bacillus cereus ceramide + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 23000, ultracentrifugation, SDS-PAGE, amino acid calculation Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 6.6
-
 substrate: insoluble long-chain phosphatidylcholine Bacillus cereus
3.1.4.3 7.5 8 substrate: water-soluble short-chain phosphatidylcholine Bacillus cereus
3.1.4.3 8
-
 substrate: insoluble long-chain phosphatidylcholine Bacillus cereus