BRENDA - Enzyme Database

Phospholipase C from Bacillus cereus

Little, C.; Methods Enzymol. 71, 725-730 (1981)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.4.3
-
Bacillus cereus
General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
urea, PLC is catalytically active in 8 M urea
Bacillus cereus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Cd2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
Cu2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
Ni2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
o-phenanthroline
-
Bacillus cereus
3.1.4.3
Urea
denaturation
Bacillus cereus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
2 mol Zn2+ per mol enzyme; metalloenzyme
Bacillus cereus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
ultracentrifugation, SDS-PAGE, amino acid calculation
Bacillus cereus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
Bacillus cereus
-
ceramide + phosphorylcholine
-
Bacillus cereus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Bacillus cereus
-
strain ATCC 10987 AB-1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
affinity chromatography steps, column chromatography
Bacillus cereus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.3
1510
-
-
Bacillus cereus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20░C, 1-2 mg purified enzyme/ml, indefinitely stable
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
-
135299
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135299
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylinositol + H2O
-
135299
Bacillus cereus
inositol monophosphate + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135299
Bacillus cereus
phosphorylserine + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
135299
Bacillus cereus
ceramide + phosphorylcholine
-
135299
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath
135299
Bacillus cereus
ceramide + phosphorylcholine
-
135299
Bacillus cereus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
23000, ultracentrifugation, SDS-PAGE, amino acid calculation
Bacillus cereus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
6.6
-
substrate: insoluble long-chain phosphatidylcholine
Bacillus cereus
3.1.4.3
7.5
8
substrate: water-soluble short-chain phosphatidylcholine
Bacillus cereus
3.1.4.3
8
-
substrate: insoluble long-chain phosphatidylcholine
Bacillus cereus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.4.3
-
Bacillus cereus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Bacillus cereus
3.1.4.3
urea, PLC is catalytically active in 8 M urea
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Cd2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
Cu2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
Ni2+
in absence of free Zn2+ in solution
Bacillus cereus
3.1.4.3
o-phenanthroline
-
Bacillus cereus
3.1.4.3
Urea
denaturation
Bacillus cereus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Zn2+
2 mol Zn2+ per mol enzyme; metalloenzyme
Bacillus cereus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
23000
-
ultracentrifugation, SDS-PAGE, amino acid calculation
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
Bacillus cereus
-
ceramide + phosphorylcholine
-
Bacillus cereus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
affinity chromatography steps, column chromatography
Bacillus cereus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.3
1510
-
-
Bacillus cereus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20░C, 1-2 mg purified enzyme/ml, indefinitely stable
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
-
135299
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135299
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylinositol + H2O
-
135299
Bacillus cereus
inositol monophosphate + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
phosphatidylserine + H2O
-
135299
Bacillus cereus
phosphorylserine + diacylglycerol
-
135299
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
135299
Bacillus cereus
ceramide + phosphorylcholine
-
135299
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath
135299
Bacillus cereus
ceramide + phosphorylcholine
-
135299
Bacillus cereus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
23000, ultracentrifugation, SDS-PAGE, amino acid calculation
Bacillus cereus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
6.6
-
substrate: insoluble long-chain phosphatidylcholine
Bacillus cereus
3.1.4.3
7.5
8
substrate: water-soluble short-chain phosphatidylcholine
Bacillus cereus
3.1.4.3
8
-
substrate: insoluble long-chain phosphatidylcholine
Bacillus cereus