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Literature summary extracted from

  • Takahashi, T.; Sugahara, T.; Ohsaka, A.
    Phospholipase C from Clostridium perfringens (1981), Methods Enzymol., 71, 710-725.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sodium cholate
-
 Clostridium perfringens
3.1.4.3 sodium dehydrocholate
-
 Clostridium perfringens

General Stability

EC Number General Stability Organism
3.1.4.3 1,10-phenanthroline, denaturation Clostridium perfringens
3.1.4.3 sodium dodecyl sulfate Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 cysteine
-
 Clostridium perfringens
3.1.4.3 EDTA
-
 Clostridium perfringens
3.1.4.3 ferricyanide
-
 Clostridium perfringens
3.1.4.3 fluoride
-
 Clostridium perfringens
3.1.4.3 glutathione
-
 Clostridium perfringens
3.1.4.3 o-phenanthroline
-
 Clostridium perfringens
3.1.4.3 phosphinate analogue of lecithin competitive inhibitor Clostridium perfringens
3.1.4.3 phosphonate analogue of lecithin competitive inhibitor Clostridium perfringens
3.1.4.3 putrescine
-
 Clostridium perfringens
3.1.4.3 SDS
-
 Clostridium perfringens
3.1.4.3 spermidine
-
 Clostridium perfringens
3.1.4.3 spermine
-
 Clostridium perfringens
3.1.4.3 thioglycolic acid
-
 Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.016 1.2 lecithin
-
 Clostridium perfringens
3.1.4.3 200
-
 p-Nitrophenylphosphorylcholine
-
 Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Clostridium perfringens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ca2+
-
 Clostridium perfringens
3.1.4.3 Co2+
-
 Clostridium perfringens
3.1.4.3 Mg2+
-
 Clostridium perfringens
3.1.4.3 Mn2+
-
 Clostridium perfringens
3.1.4.3 Zn2+
-
 Clostridium perfringens
3.1.4.3 Zn2+ metalloenzyme Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 31000
-
 gel filtration Clostridium perfringens
3.1.4.3 43000
-
 SDS-PAGE Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 1,2-diacyl-sn-glycero-3-phosphatide + H2O Clostridium perfringens
-
 1,2-diacylglycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Clostridium perfringens
-
-
-
3.1.4.3 Clostridium perfringens PB6K
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 affinity chromatography, gel filtration, 2500fold purification from culture filtrate Clostridium perfringens
3.1.4.3 column chromatography, gel filtration Clostridium perfringens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 1620
-
 substrate: lecithin Clostridium perfringens

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C, 70 mg crude enzyme/ml, stable for at least a year Clostridium perfringens
3.1.4.3 -20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months Clostridium perfringens
3.1.4.3 -20°C, stable for more than 6 months, crude enzyme Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
 Clostridium perfringens 1,2-diacylglycerol + phosphate
-
 ?
3.1.4.3 ceramide aminoethylphosphonate
-
 Clostridium perfringens N-acylsphingosine + aminoethylphosphonate
-
 ?
3.1.4.3 ceramide phosphorylethanolamine
-
 Clostridium perfringens N-acylsphingosine + phosphorylethanolamine
-
 ?
3.1.4.3 choline plasmalogen
-
 Clostridium perfringens ?
-
 ?
3.1.4.3 lysophosphatidylcholine + H2O
-
 Clostridium perfringens monoacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 lysophosphatidylcholine + H2O
-
 Clostridium perfringens PB6K monoacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 p-nitrophenylphosphoryl-choline + H2O
-
 Clostridium perfringens p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Clostridium perfringens 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Clostridium perfringens PB6K 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Clostridium perfringens phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Clostridium perfringens PB6K phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Clostridium perfringens phosphorylserine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Clostridium perfringens PB6K phosphorylserine + diacylglycerol
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Clostridium perfringens ceramide + phosphorylcholine
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Clostridium perfringens PB6K ceramide + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 31000-43000, gel filtration, SDS-PAGE Clostridium perfringens
3.1.4.3 polymer identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms Clostridium perfringens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 37
-
 for 60 min, pH 5.0-10.0 Clostridium perfringens
3.1.4.3 60 70 deactivation Clostridium perfringens
3.1.4.3 100
-
 almost complete restoration of activity of enzyme deactivated at 60-70°C Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 7 8 substrate: phosphatidylcholine Clostridium perfringens