BRENDA - Enzyme Database

Phospholipase C from Clostridium perfringens

Takahashi, T.; Sugahara, T.; Ohsaka, A.; Methods Enzymol. 71, 710-725 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium cholate
-
Clostridium perfringens
3.1.4.3
sodium dehydrocholate
-
Clostridium perfringens
General Stability
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Clostridium perfringens
3.1.4.3
sodium dodecyl sulfate
Clostridium perfringens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
cysteine
-
Clostridium perfringens
3.1.4.3
EDTA
-
Clostridium perfringens
3.1.4.3
ferricyanide
-
Clostridium perfringens
3.1.4.3
fluoride
-
Clostridium perfringens
3.1.4.3
glutathione
-
Clostridium perfringens
3.1.4.3
o-phenanthroline
-
Clostridium perfringens
3.1.4.3
phosphinate analogue of lecithin
competitive inhibitor
Clostridium perfringens
3.1.4.3
phosphonate analogue of lecithin
competitive inhibitor
Clostridium perfringens
3.1.4.3
putrescine
-
Clostridium perfringens
3.1.4.3
SDS
-
Clostridium perfringens
3.1.4.3
spermidine
-
Clostridium perfringens
3.1.4.3
spermine
-
Clostridium perfringens
3.1.4.3
thioglycolic acid
-
Clostridium perfringens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.016
1.2
lecithin
-
Clostridium perfringens
3.1.4.3
200
-
p-Nitrophenylphosphorylcholine
-
Clostridium perfringens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Clostridium perfringens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Clostridium perfringens
3.1.4.3
Co2+
-
Clostridium perfringens
3.1.4.3
Mg2+
-
Clostridium perfringens
3.1.4.3
Mn2+
-
Clostridium perfringens
3.1.4.3
Zn2+
; metalloenzyme
Clostridium perfringens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
31000
-
gel filtration
Clostridium perfringens
3.1.4.3
43000
-
SDS-PAGE
Clostridium perfringens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
1,2-diacyl-sn-glycero-3-phosphatide + H2O
Clostridium perfringens
-
1,2-diacylglycerol + phosphorylalcohol
-
Clostridium perfringens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Clostridium perfringens
-
-
-
3.1.4.3
Clostridium perfringens PB6K
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
affinity chromatography, gel filtration, 2500fold purification from culture filtrate; column chromatography, gel filtration
Clostridium perfringens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
1620
-
substrate: lecithin
Clostridium perfringens
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, 70 mg crude enzyme/ml, stable for at least a year
Clostridium perfringens
3.1.4.3
-20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months
Clostridium perfringens
3.1.4.3
-20°C, stable for more than 6 months, crude enzyme
Clostridium perfringens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
135298
Clostridium perfringens
1,2-diacylglycerol + phosphorylalcohol
-
135298
Clostridium perfringens
?
3.1.4.3
ceramide aminoethylphosphonate
-
135298
Clostridium perfringens
N-acylsphingosine + aminoethylphosphonate
-
135298
Clostridium perfringens
?
3.1.4.3
ceramide phosphorylethanolamine
-
135298
Clostridium perfringens
N-acylsphingosine + phosphorylethanolamine
-
135298
Clostridium perfringens
?
3.1.4.3
choline plasmalogen
-
135298
Clostridium perfringens
?
-
-
-
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135298
Clostridium perfringens
monoacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135298
Clostridium perfringens PB6K
monoacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
p-nitrophenylphosphoryl-choline + H2O
-
135298
Clostridium perfringens
p-nitrophenol + phosphorylcholine
-
-
-
-
3.1.4.3
phosphatidylcholine + H2O
-
135298
Clostridium perfringens
1,2-diacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
135298
Clostridium perfringens PB6K
1,2-diacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135298
Clostridium perfringens
phosphorylethanolamine + diacylglycerol
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135298
Clostridium perfringens PB6K
phosphorylethanolamine + diacylglycerol
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
phosphatidylserine + H2O
-
135298
Clostridium perfringens
phosphorylserine + diacylglycerol
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylserine + H2O
-
135298
Clostridium perfringens PB6K
phosphorylserine + diacylglycerol
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
sphingomyelin + H2O
-
135298
Clostridium perfringens
ceramide + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
sphingomyelin + H2O
-
135298
Clostridium perfringens PB6K
ceramide + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
31000-43000, gel filtration, SDS-PAGE
Clostridium perfringens
3.1.4.3
polymer
identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms
Clostridium perfringens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
37
-
for 60 min, pH 5.0-10.0
Clostridium perfringens
3.1.4.3
60
70
deactivation
Clostridium perfringens
3.1.4.3
100
-
almost complete restoration of activity of enzyme deactivated at 60-70°C
Clostridium perfringens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7
8
substrate: phosphatidylcholine
Clostridium perfringens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
sodium cholate
-
Clostridium perfringens
3.1.4.3
sodium dehydrocholate
-
Clostridium perfringens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
1,10-phenanthroline, denaturation
Clostridium perfringens
3.1.4.3
sodium dodecyl sulfate
Clostridium perfringens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
cysteine
-
Clostridium perfringens
3.1.4.3
EDTA
-
Clostridium perfringens
3.1.4.3
ferricyanide
-
Clostridium perfringens
3.1.4.3
fluoride
-
Clostridium perfringens
3.1.4.3
glutathione
-
Clostridium perfringens
3.1.4.3
o-phenanthroline
-
Clostridium perfringens
3.1.4.3
phosphinate analogue of lecithin
competitive inhibitor
Clostridium perfringens
3.1.4.3
phosphonate analogue of lecithin
competitive inhibitor
Clostridium perfringens
3.1.4.3
putrescine
-
Clostridium perfringens
3.1.4.3
SDS
-
Clostridium perfringens
3.1.4.3
spermidine
-
Clostridium perfringens
3.1.4.3
spermine
-
Clostridium perfringens
3.1.4.3
thioglycolic acid
-
Clostridium perfringens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.016
1.2
lecithin
-
Clostridium perfringens
3.1.4.3
200
-
p-Nitrophenylphosphorylcholine
-
Clostridium perfringens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Clostridium perfringens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Clostridium perfringens
3.1.4.3
Co2+
-
Clostridium perfringens
3.1.4.3
Mg2+
-
Clostridium perfringens
3.1.4.3
Mn2+
-
Clostridium perfringens
3.1.4.3
Zn2+
; metalloenzyme
Clostridium perfringens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
31000
-
gel filtration
Clostridium perfringens
3.1.4.3
43000
-
SDS-PAGE
Clostridium perfringens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
1,2-diacyl-sn-glycero-3-phosphatide + H2O
Clostridium perfringens
-
1,2-diacylglycerol + phosphorylalcohol
-
Clostridium perfringens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
affinity chromatography, gel filtration, 2500fold purification from culture filtrate; column chromatography, gel filtration
Clostridium perfringens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
1620
-
substrate: lecithin
Clostridium perfringens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20°C, 70 mg crude enzyme/ml, stable for at least a year
Clostridium perfringens
3.1.4.3
-20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months
Clostridium perfringens
3.1.4.3
-20°C, stable for more than 6 months, crude enzyme
Clostridium perfringens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
135298
Clostridium perfringens
1,2-diacylglycerol + phosphorylalcohol
-
135298
Clostridium perfringens
?
3.1.4.3
ceramide aminoethylphosphonate
-
135298
Clostridium perfringens
N-acylsphingosine + aminoethylphosphonate
-
135298
Clostridium perfringens
?
3.1.4.3
ceramide phosphorylethanolamine
-
135298
Clostridium perfringens
N-acylsphingosine + phosphorylethanolamine
-
135298
Clostridium perfringens
?
3.1.4.3
choline plasmalogen
-
135298
Clostridium perfringens
?
-
-
-
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135298
Clostridium perfringens
monoacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135298
Clostridium perfringens PB6K
monoacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
p-nitrophenylphosphoryl-choline + H2O
-
135298
Clostridium perfringens
p-nitrophenol + phosphorylcholine
-
-
-
-
3.1.4.3
phosphatidylcholine + H2O
-
135298
Clostridium perfringens
1,2-diacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
135298
Clostridium perfringens PB6K
1,2-diacylglycerol + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135298
Clostridium perfringens
phosphorylethanolamine + diacylglycerol
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135298
Clostridium perfringens PB6K
phosphorylethanolamine + diacylglycerol
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
phosphatidylserine + H2O
-
135298
Clostridium perfringens
phosphorylserine + diacylglycerol
-
135298
Clostridium perfringens
?
3.1.4.3
phosphatidylserine + H2O
-
135298
Clostridium perfringens PB6K
phosphorylserine + diacylglycerol
-
135298
Clostridium perfringens PB6K
?
3.1.4.3
sphingomyelin + H2O
-
135298
Clostridium perfringens
ceramide + phosphorylcholine
-
135298
Clostridium perfringens
?
3.1.4.3
sphingomyelin + H2O
-
135298
Clostridium perfringens PB6K
ceramide + phosphorylcholine
-
135298
Clostridium perfringens PB6K
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
31000-43000, gel filtration, SDS-PAGE
Clostridium perfringens
3.1.4.3
polymer
identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms
Clostridium perfringens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.3
37
-
for 60 min, pH 5.0-10.0
Clostridium perfringens
3.1.4.3
60
70
deactivation
Clostridium perfringens
3.1.4.3
100
-
almost complete restoration of activity of enzyme deactivated at 60-70°C
Clostridium perfringens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7
8
substrate: phosphatidylcholine
Clostridium perfringens