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Literature summary extracted from

  • Baine, W.B.
    A phospholipase C fron the Dallas IE strain of Legionella pneumophila serogroup. V. Purification and characterization of conditions for optimal activity with an artificial substrate (1988), J. Gen. Microbiol., 134, 489-498.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sorbitol
-
 Legionella pneumophila
3.1.4.3 Triton X-100
-
 Legionella pneumophila
3.1.4.3 Tween 20
-
 Legionella pneumophila
3.1.4.3 Tween 80
-
 Legionella pneumophila

General Stability

EC Number General Stability Organism
3.1.4.3 sodium dodecyl sulfate Legionella pneumophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Cu2+
-
 Legionella pneumophila
3.1.4.3 EDTA
-
 Legionella pneumophila
3.1.4.3 Fe2+
-
 Legionella pneumophila
3.1.4.3 SDS
-
 Legionella pneumophila
3.1.4.3 Zn2+
-
 Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 35 61 p-Nitrophenylphosphorylcholine
-
 Legionella pneumophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Legionella pneumophila
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ba2+ restores activity after EDTA treatment Legionella pneumophila
3.1.4.3 Ca2+ restores activity after EDTA treatment Legionella pneumophila
3.1.4.3 Co2+ restores activity after EDTA treatment Legionella pneumophila
3.1.4.3 Mg2+ restores activity after EDTA treatment Legionella pneumophila
3.1.4.3 Mn2+ restores activity after EDTA treatment Legionella pneumophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 50000 54000 SDS-PAGE Legionella pneumophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Legionella pneumophila
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Legionella pneumophila
-
 Dallas 1E strain, serogroup 5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 ion-exchange chromatography, fractionation by manganous chloride, ammonium sulfate precipitation steps Legionella pneumophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 0.029
-
 substrate: p-nitrophenylphosphorylcholine Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dipalmitoylphosphatidylcholine + H2O
-
 Legionella pneumophila 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Legionella pneumophila p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Legionella pneumophila 1,2-diacylglycerol + phosphorylcholine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 8.4
-
 substrate: p-nitrophenylphosphorylcholine Legionella pneumophila