BRENDA - Enzyme Database

Characterization of a phospholipase C activity regulated by the purified Gh in reconstitution systems

Im, M.J.; Gray, C.; Rim, A.J.; J. Biol. Chem. 267, 8887-8894 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
guanine nucleotide-binding regulatory protein Gh
stimulating only in presence of GTPgammaS, lowers required Ca2+ concentration
Rattus norvegicus
3.1.4.3
guanine nucleotide-binding regulatory protein Ghalpha
stimulation in presence of aluminium fluoride, lowers required Ca2+ concentration
Rattus norvegicus
3.1.4.3
phosphatidylcholine
-
Rattus norvegicus
3.1.4.3
phosphatidylserine
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Ca2+
activity rapidly decreases at concentrations of 0.015-0.030 mM and above
Rattus norvegicus
3.1.4.3
Mg2+
1-3 mM, substrate in detergent solution
Rattus norvegicus
3.1.4.3
phosphatidylethanolamine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
membrane
-
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Rattus norvegicus
3.1.4.3
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylinositol-bisphosphate + H2O
Rattus norvegicus
-
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
purification of Gh-PLC complex, gel filtration, ion-exchange chromatography
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
3
5
substrate: phosphatidylinositol-4,5-bisphosphate
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-70°C, buffer containing 5% glycerol and 0,1% sucrose monolaureate, stable for less than 2 weeks
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylinositol-bisphosphate + H2O
-
135291
Rattus norvegicus
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
135291
Rattus norvegicus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
guanine nucleotide-binding regulatory protein Gh
stimulating only in presence of GTPgammaS, lowers required Ca2+ concentration
Rattus norvegicus
3.1.4.3
guanine nucleotide-binding regulatory protein Ghalpha
stimulation in presence of aluminium fluoride, lowers required Ca2+ concentration
Rattus norvegicus
3.1.4.3
phosphatidylcholine
-
Rattus norvegicus
3.1.4.3
phosphatidylserine
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Ca2+
activity rapidly decreases at concentrations of 0.015-0.030 mM and above
Rattus norvegicus
3.1.4.3
Mg2+
1-3 mM, substrate in detergent solution
Rattus norvegicus
3.1.4.3
phosphatidylethanolamine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
membrane
-
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Rattus norvegicus
3.1.4.3
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylinositol-bisphosphate + H2O
Rattus norvegicus
-
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
purification of Gh-PLC complex, gel filtration, ion-exchange chromatography
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
3
5
substrate: phosphatidylinositol-4,5-bisphosphate
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-70°C, buffer containing 5% glycerol and 0,1% sucrose monolaureate, stable for less than 2 weeks
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylinositol-bisphosphate + H2O
-
135291
Rattus norvegicus
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
135291
Rattus norvegicus
?