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Literature summary extracted from

  • Im, M.J.; Gray, C.; Rim, A.J.
    Characterization of a phospholipase C activity regulated by the purified Gh in reconstitution systems (1992), J. Biol. Chem., 267, 8887-8894.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 guanine nucleotide-binding regulatory protein Gh stimulating only in presence of GTPgammaS, lowers required Ca2+ concentration Rattus norvegicus
3.1.4.3 guanine nucleotide-binding regulatory protein Ghalpha stimulation in presence of aluminium fluoride, lowers required Ca2+ concentration Rattus norvegicus
3.1.4.3 phosphatidylcholine
-
 Rattus norvegicus
3.1.4.3 phosphatidylserine
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Ca2+ activity rapidly decreases at concentrations of 0.015-0.030 mM and above Rattus norvegicus
3.1.4.3 Mg2+ 1-3 mM, substrate in detergent solution Rattus norvegicus
3.1.4.3 phosphatidylethanolamine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ca2+
-
 Rattus norvegicus
3.1.4.3 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylinositol-bisphosphate + H2O Rattus norvegicus
-
 inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 purification of Gh-PLC complex, gel filtration, ion-exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 3 5 substrate: phosphatidylinositol-4,5-bisphosphate Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -70°C, buffer containing 5% glycerol and 0,1% sucrose monolaureate, stable for less than 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylinositol-bisphosphate + H2O
-
 Rattus norvegicus inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?