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Literature summary extracted from

  • Alam, S.; Banno, Y.; Nozawa, Y.
    Purification and characterization of phospholipase C preferentially hydrolysing phosphatidylcholine in Tetrahymena membranes (1993), J. Eukaryot. Microbiol., 40, 775-781.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 Nonidet P-40
-
 Tetrahymena pyriformis
3.1.4.3 sodium deoxycholate 0.05% Tetrahymena pyriformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Triton X-100
-
 Tetrahymena pyriformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.166
-
 1,2-dipalmitoylphosphatidylcholine
-
 Tetrahymena pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 membrane
-
 Tetrahymena pyriformis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ca2+
-
 Tetrahymena pyriformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 127000
-
 gel filtration Tetrahymena pyriformis
3.1.4.3 128000
-
 SDS-PAGE Tetrahymena pyriformis

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Tetrahymena pyriformis
-
-
-
3.1.4.3 Tetrahymena pyriformis W
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 column chromatography, gel filtration chromatography, 190fold Tetrahymena pyriformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 2.083
-
 substrate: dipalmitoylphosphatidylcholine Tetrahymena pyriformis

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -80°C Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dioleoylphosphatidylethanolamine + H2O
-
 Tetrahymena pyriformis 1,2-dioleoylglycerol + phosphorylethanolamine
-
 ?
3.1.4.3 1,2-dioleoylphosphatidylethanolamine + H2O
-
 Tetrahymena pyriformis W 1,2-dioleoylglycerol + phosphorylethanolamine
-
 ?
3.1.4.3 1,2-dipalmitoylphosphatidylcholine + H2O
-
 Tetrahymena pyriformis 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 1,2-dipalmitoylphosphatidylcholine + H2O
-
 Tetrahymena pyriformis W 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Tetrahymena pyriformis 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Tetrahymena pyriformis W 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Tetrahymena pyriformis phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Tetrahymena pyriformis W phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylinositol + H2O
-
 Tetrahymena pyriformis inositol monophosphate + diacylglycerol
-
 ?
3.1.4.3 phosphatidylinositol + H2O
-
 Tetrahymena pyriformis W inositol monophosphate + diacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 127000-128000, gel filtration, SDS-PAGE Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 7
-
 substrate: dipalmitoylphosphatidylcholine Tetrahymena pyriformis