BRENDA - Enzyme Database

Purification and characterization of phospholipase C preferentially hydrolysing phosphatidylcholine in Tetrahymena membranes

Alam, S.; Banno, Y.; Nozawa, Y.; J. Eukaryot. Microbiol. 40, 775-781 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Nonidet P-40
-
Tetrahymena pyriformis
3.1.4.3
sodium deoxycholate
0.05%
Tetrahymena pyriformis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Triton X-100
-
Tetrahymena pyriformis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.166
-
dipalmitoylphosphatidylcholine
-
Tetrahymena pyriformis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
membrane
-
Tetrahymena pyriformis
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Tetrahymena pyriformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
127000
-
gel filtration
Tetrahymena pyriformis
3.1.4.3
128000
-
SDS-PAGE
Tetrahymena pyriformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Tetrahymena pyriformis
-
-
-
3.1.4.3
Tetrahymena pyriformis W
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
column chromatography, gel filtration chromatography, 190fold
Tetrahymena pyriformis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
2.083
-
substrate: dipalmitoylphosphatidylcholine
Tetrahymena pyriformis
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-80°C
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dioleoylphosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis
1,2-dioleoylglycerol + phosphorylethanolamine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
1,2-dioleoylphosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis W
1,2-dioleoylglycerol + phosphorylethanolamine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis
1,2-dipalmitoylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis W
1,2-dipalmitoylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis
1,2-diacylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis W
1,2-diacylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis
phosphorylethanolamine + diacylglycerol
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis W
phosphorylethanolamine + diacylglycerol
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylinositol + H2O
-
135289
Tetrahymena pyriformis
inositol monophosphate + diacylglycerol
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylinositol + H2O
-
135289
Tetrahymena pyriformis W
inositol monophosphate + diacylglycerol
-
135289
Tetrahymena pyriformis W
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
127000-128000, gel filtration, SDS-PAGE
Tetrahymena pyriformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7
-
substrate: dipalmitoylphosphatidylcholine
Tetrahymena pyriformis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Nonidet P-40
-
Tetrahymena pyriformis
3.1.4.3
sodium deoxycholate
0.05%
Tetrahymena pyriformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Triton X-100
-
Tetrahymena pyriformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.166
-
dipalmitoylphosphatidylcholine
-
Tetrahymena pyriformis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
membrane
-
Tetrahymena pyriformis
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Ca2+
-
Tetrahymena pyriformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
127000
-
gel filtration
Tetrahymena pyriformis
3.1.4.3
128000
-
SDS-PAGE
Tetrahymena pyriformis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
column chromatography, gel filtration chromatography, 190fold
Tetrahymena pyriformis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
2.083
-
substrate: dipalmitoylphosphatidylcholine
Tetrahymena pyriformis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-80°C
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dioleoylphosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis
1,2-dioleoylglycerol + phosphorylethanolamine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
1,2-dioleoylphosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis W
1,2-dioleoylglycerol + phosphorylethanolamine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis
1,2-dipalmitoylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
1,2-dipalmitoylphosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis W
1,2-dipalmitoylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis
1,2-diacylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylcholine + H2O
-
135289
Tetrahymena pyriformis W
1,2-diacylglycerol + phosphorylcholine
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis
phosphorylethanolamine + diacylglycerol
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135289
Tetrahymena pyriformis W
phosphorylethanolamine + diacylglycerol
-
135289
Tetrahymena pyriformis W
?
3.1.4.3
phosphatidylinositol + H2O
-
135289
Tetrahymena pyriformis
inositol monophosphate + diacylglycerol
-
135289
Tetrahymena pyriformis
?
3.1.4.3
phosphatidylinositol + H2O
-
135289
Tetrahymena pyriformis W
inositol monophosphate + diacylglycerol
-
135289
Tetrahymena pyriformis W
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.3
monomer
127000-128000, gel filtration, SDS-PAGE
Tetrahymena pyriformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7
-
substrate: dipalmitoylphosphatidylcholine
Tetrahymena pyriformis