BRENDA - Enzyme Database

Characterization of a phospholipase C produced by Pseudomaonas fluorescens

Ivanov, A.; Titibali, R.W.; Kostadinova, S.; New Microbiol. 19, 113-121 (1996)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
cetyltrimethylammonium bromide
-
Pseudomonas fluorescens
3.1.4.3
sodium deoxycholate
-
Pseudomonas fluorescens
3.1.4.3
Triton X-100
-
Pseudomonas fluorescens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Co2+
-
Pseudomonas fluorescens
3.1.4.3
Cu2+
-
Pseudomonas fluorescens
3.1.4.3
EDTA
-
Pseudomonas fluorescens
3.1.4.3
Mn2+
-
Pseudomonas fluorescens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas fluorescens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mg2+
-
Pseudomonas fluorescens
3.1.4.3
Zn2+
-
Pseudomonas fluorescens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
36000
-
gel filtration
Pseudomonas fluorescens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Pseudomonas fluorescens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
ultrafiltration, column chromatography, 792fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.3
63.3
-
-
Pseudomonas fluorescens
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
-20░C or 4░C, 0.3 M triethanolamine or 0.05 M Tris-HCl, pH 7, stable for up to 12 months
Pseudomonas fluorescens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dilauroylphosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
1,2-dilaureoylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
monoacylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
1,2-diacylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135286
Pseudomonas fluorescens
phosphorylethanolamine + diacylglycerol
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylinositol + H2O
-
135286
Pseudomonas fluorescens
inositol monophosphate + diacylglycerol
-
135286
Pseudomonas fluorescens
?
3.1.4.3
sphingomyelin + H2O
-
135286
Pseudomonas fluorescens
ceramide + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
6
6.3
substrate: L-a-phosphatidylcholine
Pseudomonas fluorescens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
cetyltrimethylammonium bromide
-
Pseudomonas fluorescens
3.1.4.3
sodium deoxycholate
-
Pseudomonas fluorescens
3.1.4.3
Triton X-100
-
Pseudomonas fluorescens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Co2+
-
Pseudomonas fluorescens
3.1.4.3
Cu2+
-
Pseudomonas fluorescens
3.1.4.3
EDTA
-
Pseudomonas fluorescens
3.1.4.3
Mn2+
-
Pseudomonas fluorescens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas fluorescens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mg2+
-
Pseudomonas fluorescens
3.1.4.3
Zn2+
-
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
36000
-
gel filtration
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
ultrafiltration, column chromatography, 792fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.3
63.3
-
-
Pseudomonas fluorescens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
-20░C or 4░C, 0.3 M triethanolamine or 0.05 M Tris-HCl, pH 7, stable for up to 12 months
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
1,2-dilauroylphosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
1,2-dilaureoylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
monoacylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylcholine + H2O
-
135286
Pseudomonas fluorescens
1,2-diacylglycerol + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135286
Pseudomonas fluorescens
phosphorylethanolamine + diacylglycerol
-
135286
Pseudomonas fluorescens
?
3.1.4.3
phosphatidylinositol + H2O
-
135286
Pseudomonas fluorescens
inositol monophosphate + diacylglycerol
-
135286
Pseudomonas fluorescens
?
3.1.4.3
sphingomyelin + H2O
-
135286
Pseudomonas fluorescens
ceramide + phosphorylcholine
-
135286
Pseudomonas fluorescens
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
6
6.3
substrate: L-a-phosphatidylcholine
Pseudomonas fluorescens