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Literature summary extracted from

  • Unemoto, T.; Hayashi, M.
    Chloride ion as a modifier of 2,3-cyclic phosphodiesterase purified from halophilic Vibrio alginolyticus (1969), Biochim. Biophys. Acta, 171, 89-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.16 Br- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 CH3COO- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 Cl- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 HCOO- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 I- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 additional information monovalent cations activate in the order of decreasing effectiveness: Cl-, Br-/I-, NO3-, HCOO-, CH3COO- Vibrio alginolyticus
3.1.4.16 NO3- activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Cd2+ 0.2 mM, 30% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 20% inhibition of 3'-AMP hydrolysis Vibrio alginolyticus
3.1.4.16 Hg2+ 0.2 mM, 35% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 36% inhibition of 3'-AMP hydrolysis Vibrio alginolyticus
3.1.4.16 MgCl2 maximal inhibition at 20 mM Vibrio alginolyticus
3.1.4.16 MgSO4 slight inhibition Vibrio alginolyticus
3.1.4.16 Na2SO4 slight inhibition Vibrio alginolyticus
3.1.4.16 Zn2+ 0.2 mM, 90% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 78% inhibition of 3'-AMP hydrolysis Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.16 0.01
-
 3'-CMP
-
 Vibrio alginolyticus
3.1.4.16 0.05
-
 3'-UMP
-
 Vibrio alginolyticus
3.1.4.16 0.08
-
 3'-AMP
-
 Vibrio alginolyticus
3.1.4.16 0.1
-
 3'-GMP
-
 Vibrio alginolyticus
3.1.4.16 0.1
-
 cyclic 2',3'-CMP
-
 Vibrio alginolyticus
3.1.4.16 0.2
-
 di-p-nitrophenyl phosphate with 200 mM NaCl Vibrio alginolyticus
3.1.4.16 0.36
-
 cyclic 2',3'-UMP
-
 Vibrio alginolyticus
3.1.4.16 0.45
-
 di-p-nitrophenyl phosphate without NaCl Vibrio alginolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.16 additional information results might indicate a surface localization Vibrio alginolyticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 Mn2+ 0.2 mM, 6-10% stimulation Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.16 65000
-
 gel filtration Vibrio alginolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Vibrio alginolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.16
-
 Vibrio alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.16 additional information
-
-
 Vibrio alginolyticus

Storage Stability

EC Number Storage Stability Organism
3.1.4.16 -20°C, pH 8.0, 20 mM Tris buffer, stable for at least 6 months Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 3'-AMP + H2O
-
 Vibrio alginolyticus adenosine + phosphate
-
 ?
3.1.4.16 3'-CMP + H2O
-
 Vibrio alginolyticus cytidine + phosphate
-
 ?
3.1.4.16 3'-GMP + H2O
-
 Vibrio alginolyticus guanosine + phosphate
-
 ?
3.1.4.16 3'-UMP + H2O
-
 Vibrio alginolyticus uridine + phosphate
-
 ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Vibrio alginolyticus 3'-CMP
-
 ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Vibrio alginolyticus 3'-GMP
-
 ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Vibrio alginolyticus 3'-UMP
-
 ?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
 Vibrio alginolyticus p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.4.16 additional information
-
 Vibrio alginolyticus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.16 55
-
 5 min, in absence of salts, 55% loss of activity, in presence of 0.2 mM MnCl2 13% loss of activity Vibrio alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 7.6
-
 hydrolysis of 3'-UMP Vibrio alginolyticus
3.1.4.16 7.8
-
 hydrolysis of 3'-CMP Vibrio alginolyticus
3.1.4.16 8.1
-
 hydrolysis of 3'-GMP Vibrio alginolyticus
3.1.4.16 8.3
-
 hydrolysis of 3'-AMP or di-p-nitrophenyl phosphate Vibrio alginolyticus
3.1.4.16 8.4
-
 cyclic phosphodiesterase activity with cyclic 2',3'-phosphate Vibrio alginolyticus
3.1.4.16 8.6
-
 cyclic phosphodiesterase activity with cyclic 2',3'-phosphate Vibrio alginolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.16 7.2 9 pH 7.2: about 45% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 3'-GMP Vibrio alginolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.16 5
-
 37°C, loss of activity below Vibrio alginolyticus
3.1.4.16 6
-
 stable for at least 2 h at 37°C Vibrio alginolyticus