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Literature summary extracted from

  • Center, M.S.; Behal, F.J.
    A cyclic phosphodiesterase with 3-nucleotidase activity from Proteus mirabilis (1968), J. Biol. Chem., 243, 138-143.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.16 chymotrypsin, 10 min at 37°C, 80% loss of phosphodiesterase activity and 3'-nucleotidase activity Proteus mirabilis
3.1.4.16 trypsin, 10 min at 37°C, 50% loss of phosphodiesterase activity Proteus mirabilis
3.1.4.16 urea, 6.4 M, 53% loss of activity after 10 min, 20% loss of activity after 20 min Proteus mirabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Zn2+
-
 Proteus mirabilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 KCN slight activation of cyclic phosphodiesterase Proteus mirabilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Proteus mirabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.16 partial Proteus mirabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 3'-AMP + H2O
-
 Proteus mirabilis adenosine + phosphate
-
 ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Proteus mirabilis 3'-CMP
-
 ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Proteus mirabilis 3'-GMP
-
 ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Proteus mirabilis 3'-UMP
-
 ?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
 Proteus mirabilis p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Proteus mirabilis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 7 8
-
 Proteus mirabilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.16 6 9 about 55% of maximal activity at pH 6 and at pH 9 Proteus mirabilis