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Literature summary extracted from
- Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli (1990), J. Bacteriol., 172, 5445-5449.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.14 | CHAPS | - |
Escherichia coli |  |
| 3.1.4.14 | Triton X-100 | biphasic, optimal stimulation at 1 mM | Escherichia coli | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.14 | 90°C, 1% SDS, 10 min, 20% loss of activity | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.14 | SDS | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.14 | Mg2+ | - |
Escherichia coli | |
| 3.1.4.14 | Mn2+ | increased activity in combination with Mg2+ | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.14 | 25000 | - |
x * 25000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.14 | acyl-carrier protein + H2O | Escherichia coli | - |
4'-phosphopantetheine + apoprotein | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.14 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.14 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.14 | acyl-carrier protein + H2O | - |
Escherichia coli | 4'-phosphopantetheine + apoprotein | - |
? | |
| 3.1.4.14 | E. coli acyl-carrier protein + H2O | - |
Escherichia coli | 4'-phosphopantetheine + apoprotein of acyl-carrier protein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.14 | ? | x * 25000, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.14 | ACP phosphodiesterase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.14 | 42 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.14 | 60 | - |
loss of activity after 15 min in the absence of SDS | Escherichia coli |
| 3.1.4.14 | 90 | - |
stable in the presence of 1% SDS | Escherichia coli |
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