BRENDA - Enzyme Database

Purification and characterization of a membrane bound neutral pH optimum magnesium-dependent and phosphatidylserine-stimulated sphingomyelinase from rat brain

Liu, B.; Hassler, D.F.; Smith, G.K.; Weaver, K.; Hannun, Y.A.; J. Biol. Chem. 273, 34472-34479 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
arachidonic acid
-
Rattus norvegicus
3.1.4.12
dithiothreitol
2fold enhanced activity at 3 mM
Rattus norvegicus
3.1.4.12
lyso-phosphatidylcholine
-
Rattus norvegicus
3.1.4.12
phosphatidic acid
-
Rattus norvegicus
3.1.4.12
phosphatidylethanolamine
-
Rattus norvegicus
3.1.4.12
phosphatidylinositol
-
Rattus norvegicus
3.1.4.12
phosphatidylserine
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
Cu2+
-
Rattus norvegicus
3.1.4.12
deoxycholate
-
Rattus norvegicus
3.1.4.12
glutathione
-
Rattus norvegicus
3.1.4.12
Mg2+
slight inhibitory at higher concentrations
Rattus norvegicus
3.1.4.12
Ni2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
additional information
-
sphingomyelin
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
membrane
-
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
dependent
Rattus norvegicus
3.1.4.12
Mn2+
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
150000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.12
partial, 3030fold
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
brain
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.12
4°C, 14 days, Triton X-100
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
135183
Rattus norvegicus
N-acylsphingosine + choline phosphate
-
135183
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.12
?
x * 55000-90000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7
-
neutral sphingomyelinase
Rattus norvegicus
3.1.4.12
7.5
-
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
arachidonic acid
-
Rattus norvegicus
3.1.4.12
dithiothreitol
2fold enhanced activity at 3 mM
Rattus norvegicus
3.1.4.12
lyso-phosphatidylcholine
-
Rattus norvegicus
3.1.4.12
phosphatidic acid
-
Rattus norvegicus
3.1.4.12
phosphatidylethanolamine
-
Rattus norvegicus
3.1.4.12
phosphatidylinositol
-
Rattus norvegicus
3.1.4.12
phosphatidylserine
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
Cu2+
-
Rattus norvegicus
3.1.4.12
deoxycholate
-
Rattus norvegicus
3.1.4.12
glutathione
-
Rattus norvegicus
3.1.4.12
Mg2+
slight inhibitory at higher concentrations
Rattus norvegicus
3.1.4.12
Ni2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
additional information
-
sphingomyelin
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
membrane
-
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
dependent
Rattus norvegicus
3.1.4.12
Mn2+
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
150000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.12
partial, 3030fold
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
brain
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.12
4°C, 14 days, Triton X-100
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
135183
Rattus norvegicus
N-acylsphingosine + choline phosphate
-
135183
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.12
?
x * 55000-90000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7
-
neutral sphingomyelinase
Rattus norvegicus
3.1.4.12
7.5
-
-
Rattus norvegicus