EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | cetyltrimethylammonium bromide | low concentrations | Rattus norvegicus | |
3.1.4.12 | Detergents | - |
Rattus norvegicus | |
3.1.4.12 | Triton X-100 | - |
Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | ceramide | 23% inhibition | Rattus norvegicus | |
3.1.4.12 | cetyltrimethylammonium bromide | - |
Rattus norvegicus | |
3.1.4.12 | phosphorylcholine | 23% inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 0.5 | 0.9 | sphingomyelin | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | lysosome | - |
Rattus norvegicus | 5764 | - |
3.1.4.12 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.12 | partial, 21fold | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | liver | - |
Rattus norvegicus | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.4.12 | -18°C, 0.25 M sucrose, or 20% glycerol, or 33% (NH4)2SO4 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | erythro-isomers of DL-trans-2-N-palmitoyl-1-O-phosphorylcholinesphingosine or dihydrosphingosine hydrolyzed faster than the threo-isomer | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
3.1.4.12 | sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 65 | - |
inactive after 10 min | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 5 | 5.5 | depending on buffer | Rattus norvegicus |