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Literature summary extracted from

  • Tomita, M.; Taguchi, R.; Ikezawa, H.
    Molecular properties and kinetic studies on sphingomyelinase of Bacillus cereus (1982), Biochim. Biophys. Acta, 704, 90-99.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 Detergents
-
 Bacillus cereus
3.1.4.12 sodium deoxycholate
-
 Bacillus cereus
3.1.4.12 Triton X-100 activation with sphingomyelin Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Ca2+ when sphingomyelin as substrate with Triton X-100 is used, competitive inhibitor against Mg2+ Bacillus cereus
3.1.4.12 cetyltrimethylammonium bromide
-
 Bacillus cereus
3.1.4.12 EDTA
-
 Bacillus cereus
3.1.4.12 Triton X-100 slight inhibition with 2-hexadecynoylamino-4-nitrophenylphosphorylcholine Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.034
-
 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine in presence of Mg2+ Bacillus cereus
3.1.4.12 0.038
-
 sphingomyelin in presence of Mg2+ and Triton X-100 Bacillus cereus
3.1.4.12 0.17
-
 sphingomyelin in presence of Mg2+ and sodium deoxycholate Bacillus cereus
3.1.4.12 0.174
-
 hexadecanoyl-p-nitrophenyl phosphorylcholine
-
 Bacillus cereus
3.1.4.12 0.405
-
 sphingomyelin in presence of Triton X-100 Bacillus cereus
3.1.4.12 0.565
-
 sphingomyelin in presence of sodium deoxycholate Bacillus cereus
3.1.4.12 11.6
-
 4-nitrophenyl phosphorylcholine
-
 Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+ slight activation when water-soluble substrates are used Bacillus cereus
3.1.4.12 Mg2+
-
 Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 23300
-
 sedimentation equilibrium centrifugation Bacillus cereus
3.1.4.12 41000
-
 x * 41000, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Bacillus cereus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 2200fold Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate
-
 ?
3.1.4.12 4-nitrophenyl phosphorylcholine + H2O
-
 Bacillus cereus 4-nitrophenol + phosphorylcholine
-
 ?
3.1.4.12 hexadecanoyl-p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus hexadecanoyl-p-nitrophenol + choline phosphate
-
 ?
3.1.4.12 lysophosphatidylcholine + H2O
-
 Bacillus cereus 1-acylglycerol + choline phosphate
-
 ?
3.1.4.12 p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus choline phosphate + p-nitrophenol
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Bacillus cereus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 ? x * 41000, SDS-PAGE Bacillus cereus