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Literature summary extracted from

  • Watanabe, K.; Sakuragawa, N.; Arima, M.; Satoyoshi, E.
    Partial purification and properties of acid sphingomyelinase from rat liver (1983), J. Lipid Res., 24, 596-603.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 Detergents
-
 Rattus norvegicus
3.1.4.12 Triton X-100 activation at very low concentrations Rattus norvegicus

General Stability

EC Number General Stability Organism
3.1.4.12 glycerol, stabilization Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Chloroquine diphosphate salt, 50% inhibition at 5 mM Rattus norvegicus
3.1.4.12 dithiothreitol
-
 Rattus norvegicus
3.1.4.12 phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.027
-
 2n-hexadecanoylamino-4-nitrophenylphosphorylcholine
-
 Rattus norvegicus
3.1.4.12 0.079
-
 sphingomyelin
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 lysosome
-
 Rattus norvegicus 5764
-
3.1.4.12 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 40000
-
 1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE Rattus norvegicus
3.1.4.12 44500
-
 1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE Rattus norvegicus
3.1.4.12 45600
-
 1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE Rattus norvegicus
3.1.4.12 220000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 5200fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 additional information
-
 different assay conditions, substrates etc. Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O
-
 Rattus norvegicus 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate
-
 ?
3.1.4.12 bis(4-methylumbelliferyl)-phosphate + H2O poor substrate Rattus norvegicus ?
-
 ?
3.1.4.12 bis-p-nitrophenyl phosphate + H2O poor substrate Rattus norvegicus ?
-
 ?
3.1.4.12 hexadecanoyl-p-nitrophenylphosphorylcholine + H2O
-
 Rattus norvegicus hexadecanoyl-p-nitrophenol + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 trimer 1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 5
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.12 5
-
 Chloroquine diphosphate salt Rattus norvegicus