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Literature summary extracted from

  • Weitz, G.; Lindl, T.; Hinrichs, U.; Sandhoff, K.
    Release of sphingomyelin phosphodiesterase (acid sphingomyelinase) by ammonium chloride from CL 1D mouse L-cells and human fibroblasts. Partial purification and characterization of the exported enzymes (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 863-871.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 Triton X-100
-
 Mus musculus
3.1.4.12 Triton X-100
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.024
-
 sphingomyelin
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 extracellular in presence of NH4Cl Mus musculus
-
-
3.1.4.12 extracellular in presence of NH4Cl Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 68000
-
 gel filtration or gel electrophoresis Mus musculus
3.1.4.12 68000
-
 gel filtration or gel electrophoresis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-
3.1.4.12 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 134fold Homo sapiens
3.1.4.12 64fold Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 fibroblast
-
 Homo sapiens
-
3.1.4.12 L-cell cell line Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 additional information
-
 different assay conditions, substrates etc. Mus musculus
3.1.4.12 additional information
-
 different assay conditions, substrates etc. Homo sapiens
3.1.4.12 additional information
-
 normal and Niemann Pick disease type C fibroblasts Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.4.12 4°C, stable for several weeks Mus musculus
3.1.4.12 4°C, stable for several weeks Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.12 56
-
 20 min, 40% activity Mus musculus
3.1.4.12 56
-
 30 min, 40% activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 4.4
-
-
 Homo sapiens
3.1.4.12 4.8
-
-
 Mus musculus