BRENDA - Enzyme Database

Acid sphingomyelinase from human urine: purification and characterization

Quintern, L.E.; Weitz, G.; Nehrkorn, H.; Tager, J.M.; Schram, A.W.; Sandhoff, K.; Biochim. Biophys. Acta 922, 323-336 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
ceramide
-
Homo sapiens
3.1.4.12
Diacylglycerols
-
Homo sapiens
3.1.4.12
Lipids
saturated and unsaturated fatty acids
Homo sapiens
3.1.4.12
monoacylglycerols
-
Homo sapiens
3.1.4.12
triacylglycerols
-
Homo sapiens
3.1.4.12
Tripalmitoylglycerol
-
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.1.4.12
detergents, e.g. Nonidet P-40, stabilization
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
2'-AMP
mixture with 3'-AMP
Homo sapiens
3.1.4.12
3'-AMP
mixture with 2'-AMP
Homo sapiens
3.1.4.12
3-[benzyl(tert-butyl)amino]-1-(4-nitrophenyl)propan-1-one
-
Homo sapiens
3.1.4.12
5'-AMP
-
Homo sapiens
3.1.4.12
adenine-9-beta-D-arabinofuranoside 5'-monophosphate
-
Homo sapiens
3.1.4.12
adenosine 3',5'-diphosphate
-
Homo sapiens
3.1.4.12
adenosine 5'-monophosphate
periodate-oxidized and an isomer with arabinose instead of ribose as sugar component
Homo sapiens
3.1.4.12
Adenosine 5'-O-thiomonosulfate
-
Homo sapiens
3.1.4.12
adenosine 5'-phosphosulfate
-
Homo sapiens
3.1.4.12
dithioerythritol
-
Homo sapiens
3.1.4.12
phosphatidylinositol
-
Homo sapiens
3.1.4.12
phosphatidylinositol 4',5'-bisphosphate
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
0.069
-
sphingomyelin
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
70000
-
x * 70000, 2 or 4 subunits, SDS-PAGE
Homo sapiens
3.1.4.12
200000
-
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Homo sapiens
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.12
22990fold
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
urine
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.12
41
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.12
4°C, 50% loss of activity after 3-5 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
phosphatidylcholine + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + choline phosphate
-
-
-
?
3.1.4.12
phosphatidylethanolamine + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + phosphorylethanolamine
-
-
-
?
3.1.4.12
phosphatidylglycerol + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + glycerolphosphate
-
135149
Homo sapiens
?
3.1.4.12
phosphatidylglycerol + H2O
in the absence of detergents an in the presence of 0.05% Nonidet P-40
135149
Homo sapiens
diacylglycerol + glycerolphosphate
-
135149
Homo sapiens
?
3.1.4.12
sphingomyelin + H2O
-
135149
Homo sapiens
N-acylsphingosine + choline phosphate
-
-
-
?
3.1.4.12
sphingomyelin + H2O
-
135149
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.12
?
x * 70000, 2 or 4 subunits, SDS-PAGE
Homo sapiens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.12
7.2
-
highest stability
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
ceramide
-
Homo sapiens
3.1.4.12
Diacylglycerols
-
Homo sapiens
3.1.4.12
Lipids
saturated and unsaturated fatty acids
Homo sapiens
3.1.4.12
monoacylglycerols
-
Homo sapiens
3.1.4.12
triacylglycerols
-
Homo sapiens
3.1.4.12
Tripalmitoylglycerol
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.12
detergents, e.g. Nonidet P-40, stabilization
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
2'-AMP
mixture with 3'-AMP
Homo sapiens
3.1.4.12
3'-AMP
mixture with 2'-AMP
Homo sapiens
3.1.4.12
3-[benzyl(tert-butyl)amino]-1-(4-nitrophenyl)propan-1-one
-
Homo sapiens
3.1.4.12
5'-AMP
-
Homo sapiens
3.1.4.12
adenine-9-beta-D-arabinofuranoside 5'-monophosphate
-
Homo sapiens
3.1.4.12
adenosine 3',5'-diphosphate
-
Homo sapiens
3.1.4.12
adenosine 5'-monophosphate
periodate-oxidized and an isomer with arabinose instead of ribose as sugar component
Homo sapiens
3.1.4.12
Adenosine 5'-O-thiomonosulfate
-
Homo sapiens
3.1.4.12
adenosine 5'-phosphosulfate
-
Homo sapiens
3.1.4.12
dithioerythritol
-
Homo sapiens
3.1.4.12
phosphatidylinositol
-
Homo sapiens
3.1.4.12
phosphatidylinositol 4',5'-bisphosphate
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
0.069
-
sphingomyelin
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
70000
-
x * 70000, 2 or 4 subunits, SDS-PAGE
Homo sapiens
3.1.4.12
200000
-
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Homo sapiens
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.12
22990fold
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
urine
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.12
41
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.12
4°C, 50% loss of activity after 3-5 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
phosphatidylcholine + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + choline phosphate
-
-
-
?
3.1.4.12
phosphatidylethanolamine + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + phosphorylethanolamine
-
-
-
?
3.1.4.12
phosphatidylglycerol + H2O
in the presence of 0.08% sodium taurodeoxycholate
135149
Homo sapiens
diacylglycerol + glycerolphosphate
-
135149
Homo sapiens
?
3.1.4.12
phosphatidylglycerol + H2O
in the absence of detergents an in the presence of 0.05% Nonidet P-40
135149
Homo sapiens
diacylglycerol + glycerolphosphate
-
135149
Homo sapiens
?
3.1.4.12
sphingomyelin + H2O
-
135149
Homo sapiens
N-acylsphingosine + choline phosphate
-
-
-
?
3.1.4.12
sphingomyelin + H2O
-
135149
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.12
?
x * 70000, 2 or 4 subunits, SDS-PAGE
Homo sapiens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.12
7.2
-
highest stability
Homo sapiens