BRENDA - Enzyme Database

Expression, purification and characterisation of a functional phosphatidylinositol-specific phospholipase C-delta1 protein in Escherichia coli

Ginger, R.S.; Parker, P.J.; Eur. J. Biochem. 210, 155-160 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
phosphatidylethanolamine
stimulation in presence of 1 mM Ca2+
Homo sapiens
3.1.4.11
phosphatidylserine
stimulation in presence of 1 mM Ca2+
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.11
expression in Escherichia coli
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
phosphatidylethanolamine
25% inhibition, in presence of 0.001 mM Ca2+
Homo sapiens
3.1.4.11
phosphatidylserine
50% inhibition, in presence of 0.001 mM Ca2+
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.11
0.031
-
phosphatidylinositol 4,5-bisphosphate
-
Homo sapiens
3.1.4.11
0.031
-
phosphatidylinositol 4-phosphate
-
Homo sapiens
3.1.4.11
0.143
-
phosphatidylinositol
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
significant hydrolysis of phosphatidylinositol only above 0.1 mM
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.11
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.11
-
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
200
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
7% of the activity with phosphatidylinositol 4,5-diphosphate
135141
Homo sapiens
?
-
-
-
?
3.1.4.11
phosphatidylinositol 4,5-bisphosphate + H2O
-
135141
Homo sapiens
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
-
-
?
3.1.4.11
phosphatidylinositol 4-phosphate
20% of the activity with phosphatidylinositol 4,5-diphosphate
135141
Homo sapiens
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
7
7.5
pH 7.0: about 30% of maximal activity, pH 7.5: about 75% of maximal activity, hydrolysis of phosphatidylinositol 4,5-diphosphate
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.11
0.001
-
phosphatidylserine
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
phosphatidylethanolamine
stimulation in presence of 1 mM Ca2+
Homo sapiens
3.1.4.11
phosphatidylserine
stimulation in presence of 1 mM Ca2+
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.11
expression in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
phosphatidylethanolamine
25% inhibition, in presence of 0.001 mM Ca2+
Homo sapiens
3.1.4.11
phosphatidylserine
50% inhibition, in presence of 0.001 mM Ca2+
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.11
0.001
-
phosphatidylserine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.11
0.031
-
phosphatidylinositol 4,5-bisphosphate
-
Homo sapiens
3.1.4.11
0.031
-
phosphatidylinositol 4-phosphate
-
Homo sapiens
3.1.4.11
0.143
-
phosphatidylinositol
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
significant hydrolysis of phosphatidylinositol only above 0.1 mM
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.11
-
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
200
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
7% of the activity with phosphatidylinositol 4,5-diphosphate
135141
Homo sapiens
?
-
-
-
?
3.1.4.11
phosphatidylinositol 4,5-bisphosphate + H2O
-
135141
Homo sapiens
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
-
-
?
3.1.4.11
phosphatidylinositol 4-phosphate
20% of the activity with phosphatidylinositol 4,5-diphosphate
135141
Homo sapiens
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
7
7.5
pH 7.0: about 30% of maximal activity, pH 7.5: about 75% of maximal activity, hydrolysis of phosphatidylinositol 4,5-diphosphate
Homo sapiens